Neurodegeneration and Neurodegenerative Diseases
Neurodegeneration and Neurodegenerative DiseasesWhat do we fear the most when we age? Most of us will most like fear the degeneration of our brain power the most. I admit that symptoms like forgetting...
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Amino Acid Analysis
Amino Acid Analysis0. IntroductionThe analysis of free amino acids present in food samples, body fluids such as urine, serum and blood, and other sources, amino acid hydrolysates, from proteins, or...
View ArticleAbbreviation for Antibody Species Reactivity
<div><b><font size="4">Abbreviations</font></b></div> <table> <tbody>...
View ArticleAbbreviations for Antibody Tested Applications
AbbreviationsArArabidopsisBBovineBABaboonBcBacteriaBlBlockingBtBiotinylatedCeC. elegansCChickenChmChimpanzeeCPCarpCTC-TerminusDDogDsDrosophilaEELISAEDExtracellular DomainELExtracellular...
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Bridged nucleic Acids (BNA) - BNA3
Bridged Nucleic Acids (BNAs) - BNA3Beyond peptide nucleic acids (PNAs) and locked nucleic acids (LNAs)The near completion of the human genome sequence in 2001 as well as the availability of whole...
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Citrullinated Peptide Synthesis
Citrullinated Peptide Synthesis The amino acid citrulline is not coded for by DNA directly however several proteins are known to contain citrulline as a result of a posttranslational modification....
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Mycobacterium tuberculosis peptide libraries
Mycobacterium tuberculosis peptide librariesDesign of Mtb peptide libraries for targeted researchTuberculosis, an ancient disease, has become an escalating global health problem in recent years. It is...
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Brief history of Bridged Nucleic Acids (BNAs)
A brief history of Bridged Nucleic Acids (BNAs) A quest for better oligonucleotide mimics. The quest for oligonucleotide mimics with improved characteristics and stabilities useful for molecular...
View ArticleDNA-mediated site-specific deposition of gold nanoparticles on silicon wafers
We report a method for the site-specific deposition of gold nanoparticles, as programmed by DNA sequences immobilized on the surface of silicon oxide-coated silicon wafers. After optimization of...
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Bridged Nucleic Acid (BNA3) Design Guidelines
Design of BNANC [NMe] modified oligonucleotidesThe placing of BNA NC [NMe] monomers in oligonucleotides, both, for DNA and RNA, is shown based on examples from published experimental...
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Allele Specific BNA Primer Design
Rules for the design of allele specific primersFor regular DNA primers: Design primers that are specific to the 3’ end nucleotide of the SNP and the 3rd nucleotide from the 3’ end is mismatched with...
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Peptide libraries for heterogeneous nuclear ribonucleoproteins or hnRNPs
Heterogeneous nuclear ribonucleoproteins (hnRNPs) isoformsHeterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a family of RNA-binding proteins. The nature of hnRNPs is very complex and diverse....
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BNAs for Duplex and Triplex Formation
BNAs for Duplex and Triplex Formation2’,4’-BNA (LNA) with a five-membered bridged structure is insufficiently resistant to nucleases, and fully modified 2’,4’-BNA (LNA) oligonucleotides do not have the...
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References for BNANC[NH] and [NMe] monomers
References for BNANC[NH] and [NMe] monomersApplicationBNAPublicationYearHybridization.Nuclease stabilityDuplex-Formation RNA and DNARahman SM, Seki S, Utsuki K, Obika S, Miyashita K, Imanishi T.;...
View ArticleBuffer Used In BNA Experiments
Buffers used in BNA experimentsApplicationConcentrationBufferReferenceAntisense BNAs1 micomolar (1 µM)20 mM sodium phosphate, 100 mM NaCl pH 7.2Obika et al., 2001Duplex formationDuplex concentration 4...
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What are lncRNAs and lincRNAs
What are lncRNAs and lincRNAsRecent discoveries increasingly indicate that only a small percentage, approximately 7%, of disease-associated single nucleotide polymorphisms (SNPs) are located in...
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solid phase polypeptide synthesis
Solid Phase Polypeptide Synthesis (SPPS)The control and regulatory mechanisms for many biological processes are dependent on peptides and proteins derived from α–amino acids. In addition, many modern...
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oligonucleotides containing epigenetic cytosine modifications
Bio-Synthesis provides synthesis of oligonucleotide containing methylated DNA base for epigenetic studies . Epigenetics is the study of changes in heritage control o f gene expression through an...
View ArticlePurification and Quality Control of Long RNA Synthesis by Transcription
Purification of Synthesized Long RNA Transcripts After long RNA synthesis by transcribing from DNA template, unmodified RNA transcripts from standard DNA synthesis are been purified by...
View ArticleHeavy Isotope Labeling
Isotopically labeled peptides Stable-isotope labeled peptides are used in biomarker discovery and validation as well as in drug and metabolite monitoring, peptide signaling experiments, metabolomics...
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