Purification and Quality Control of Long RNA Synthesis by Transcription

Purification of Synthesized Long RNA Transcripts

After long RNA synthesis by transcribing from DNA template, unmodified RNA transcripts from standard DNA synthesis are been purified by phenol-chloroform extraction and ethanol precipitation following with spin column chromatography.  For capped RNA syntehsis, non-radioactively labeled RNA or highly specific activity radiolabeled RNA probes, spin column chromatography is the preferred method. When high purity RNA transcript for labeled RAN rpobes for RNase protection assay or foot printing experiments, we recommend gel purificaiton of the transcription product.

Bio-Synthesis's Long RNA Synthesis transcription service includes phenol-chloroform extraction and ethanol precipitation. Final product are further purified using spin column chromatography. Gel purification is available with an additional fee.

Characterization of RNA transcription Products

After transcription from template DNA, RNA transcripts concentration are been determined by measuring with ultraviolet light absorbance at 260 nm wavelength. Evaluation of transcript length, integrity and quantity are further analyzed on an appropriate agarose gel or polyacrylamide gel.