Peptides with Anti-Cancer Properties
Klaus D. Linse A review of the scientific biomedical literature reveals that many peptides have anti-cancer activities or properties. The following table contains a list of peptides that are reported...
View ArticlePeptide based LHRH agonists and new generation antagonists
Luteinizing hormone-releasing hormone (LH-RH or LHRH) agonists and gonadotropin-releasing hormone (GnRH) agonists are peptides or drugs that are used for hormone therapy. These drugs lower the...
View ArticlePeptide receptors are important for cancer therapies
Peptide receptors are important for cancer therapies By Klaus D. Linse Our understanding of estrogen signaling has increased significantly in recent decades. Research findings indicate that estrogen...
View ArticleClik here to view.
Molecular probes for quantitative PCR or qPCR
Molecular probes and their applications in real-time quantitative PCR (qPCR)By Andrei Laikhter The past decades have seen a remarkable growth in the use of modified oligonuleotides as tools for the...
View ArticleAmino Linker Oligonucleotide Modification
Primary alkyamino modified oligonucleotides are commonly used in oligonucleotide labeling and conjugations towards such electrophilic moieties as active esters, halogenated aliphatic and aromatic...
View ArticleBiotinylated Oligonucleotides
Biosynthesis offers several different Oligonucleotide biotinylation synthesis options. Avidin, streptavidin and other biotin-binding proteins have the ability to form an intense association with...
View ArticleClik here to view.
Real time detection of influenza virus H7N9
Real time detection of influence virus strain H7N9The development of the polymer chain reaction (PCR), for which Kary B. Mullis received a Nobel Prize in 1993 together with Michael Smith, has...
View ArticleClik here to view.
A Novel BNA Probes Bead Based Assay
A novel bead-based suspension assay using BNA-NC probes to detect and quantify somatic mutations in leukemiaA novel bead-based suspension assay using Bridged Nucleic Acid, BNA-NC, probes for the...
View ArticleClik here to view.
Acivicin, a modified amino acid that inhibits tumor growth
The amino acid acivicin [(alpha S,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid; AT-125; NSC-163501] is a fermentation product of Streptomyces sviceus. Acivicin is a modified amino acid,...
View ArticleClik here to view.
Amino Acids
SYMBOLAMINO ACIDSTRUCTUREFORMULAMOL WTMW-H2βAlaβ-AlanineC3H7NO289.0971.07AlaAlanineC3H7NO289.0971.07AadAminoadipic acidC6H11NO4161.16143.14Abu2-Aminobutyric acidC4H9NO2103.1285.104-Abz4-Aminobenzoic...
View ArticleClik here to view.
Melting Temperature (Tm) Calculation for BNA Oligonucleotides
Melting Temperature (Tm) Calculation for BNA Oligonucleotides By Klaus D. Linse Several mutation detection techniques rely on the chemical- or temperature-driven melting behavior of short...
View ArticleGene Synthesis
More than 30 years after the first complete gene, a yeast tRNA, was synthesized Har Gobind Khorana and coworkers in 1972 and the synthesis of the first peptide- and protein-coding genes was performed...
View ArticleClik here to view.
Cell Penetrating Peptide Delivers Proteins Into Cells
Cell Penetrating Peptide Delivers Proteins Into CellsBy Klaus D. Linse Cell-penetrating peptides, or CPPs, are short peptides that can facilitate cellular uptake of a variety of molecular cargo. CPPs...
View ArticlePeptide Libraries
Peptide libraries allow for the search of peptide based bioactive molecules by screening large numbers of peptides for their activity. Often, this is not possible with the help of traditional chemical...
View ArticleClik here to view.
Amino Acid Analysis of Tyramine
Amino Acid Analysis of TyramineBy Klaus LindeAmino acid analysis allows for the analysis and quantitative detection of tyramine using a standard amino acid analyzer. Amino acid analysis of Tyramine can...
View ArticleClik here to view.
Tyramide Signal Amplification
Tyramide Signal Amplification (TSA)By Klaus D. LinseTyramide Signal Amplification is a novel technique using a biotinylated tyramine to detect specific proteins or nucleic acid sequences in-situ, where...
View ArticleClik here to view.
Protein Methylation
Protein MethylationBy Klaus D. LinseProteins are controlled or regulated by a large battery of post-translational modifications (PTMs). Post-translational modification of proteins refers to biochemical...
View ArticleClik here to view.
Mechanisms of Protein Lysine and Arginine Modifications
Mechanisms of Protein Lysine and Arginine Modifications By Klaus D. LinseArginine and lysine are abundant amino acids found in basic proteins such as histones and protamines. Histones belong to the...
View ArticleQuantification of dsRNA
Gel electrophoresis: Run total of 0.5 µl of the unpurified and 1 µl of the purified dsRNA in 10 µl total volume on a 1% agarose gel. Run 2 µl low mass ladder on a side. Confirm yield and size of single...
View ArticleClik here to view.
BNA based FISH Probes
BNA based FISH probes Recent advancements in the design and development of molecular probes and image analysis has made fluorescence in situ hybridization (FISH) a powerful tool. Although being a...
View Article