Biosynthesis offers several different Oligonucleotide biotinylation synthesis options. Avidin, streptavidin and other biotin-binding proteins have the ability to form an intense association with biotin-containing molecules. This association has been used for many years to develop systems designed to capture biotinylated biomolecules. A variety of applications are in routine use to exploit the extraordinary affinity of these biotin-binding proteins for biotinylated molecules. However, the biggest bottleneck of biotin-binding proteins for biotin is that the association is essentially irreversible. Extremely low pH or highly concentrated chaotropic reagents are required to break teh association and these conditions are not entirely compatible with oligonucleotides. Biotin analogue such as desthiobiotin exhibits lower binding to biotin-binding porteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constnat (Kd) several orders of magnitude less than biotin/streptaviding. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin.
Biotinylated Oligonucleotide has been used in numerous applications, primarily, centering around the use of the biotin moiety as means to effect the avidin-mediated separation of a biotinylated oligonucleotide which has undergone hybridization with a target sequence. Biotin is also used as a way to attach avidin-conjugated enzymes used in chemiluminescent and colormetric1 detection protocols. Biotinylated oligonucleotides have been used in solid-phase differential display protocols as well as solid phase genomic and plasmid sequencing protocols such as solid phase capture by Streptavidin coated magnetic beads for use in restriction mapping,2 PCR based genomic walking,3 differential display detection of unique mRNA species,4 and DNA sequencing.5 5’- And 3’-biotinilated oligonucleotides as well as multi biotinilated oligonucleotides can be used for those applications.
Oligonucleotide biotin modification includes:
Contact us for Biotinlyated Oligonucleotide Synthesis Services.
References:
Biotinylated Oligonucleotide has been used in numerous applications, primarily, centering around the use of the biotin moiety as means to effect the avidin-mediated separation of a biotinylated oligonucleotide which has undergone hybridization with a target sequence. Biotin is also used as a way to attach avidin-conjugated enzymes used in chemiluminescent and colormetric1 detection protocols. Biotinylated oligonucleotides have been used in solid-phase differential display protocols as well as solid phase genomic and plasmid sequencing protocols such as solid phase capture by Streptavidin coated magnetic beads for use in restriction mapping,2 PCR based genomic walking,3 differential display detection of unique mRNA species,4 and DNA sequencing.5 5’- And 3’-biotinilated oligonucleotides as well as multi biotinilated oligonucleotides can be used for those applications.
Oligonucleotide biotin modification includes:
- 5' Biotin
- Biotin dT
- Photocleavable PC Biotin
- Desthiobiotin
- 3' Biotin
Contact us for Biotinlyated Oligonucleotide Synthesis Services.
References:
- Misra, R. R.; Chiang, S. Y.; Swenberg, J. A. Carcinogenesis, 1994, 15, 1647.
- Conrad, F.; Krupp, G., Nucleic Acids Res., 1992, 20, 6423.
- Rosenthal, A.; Jones, D. S. C., Nucleic Acids Res., 1990, 18, 3095.
- Rostok, O.; Odeberg, J.; Rode, M.; Stokke, T.; Fundruck, S.; Smeland, E.; Lunderberg, J., Biotechniques, 1996, 21, 114.
- Huitman, T.; Stahl, S.; Hornes, E.; Uhlen, M., Nucleic Acids Res., 1989, 17, 4937.