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Exploring the efficacy of combination therapy for DNA crosslinking drug...
The recent surge in interest in herbal medicine stems from an increased curiosity in determining whether the efficacy of anticancer drugs could be augmented via additional ingredients. In the case of...
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FDA approves first digital PCR liquid biopsy test employing oligonucleotide...
For a considerable period, quantitative PCR (pPCR) has been utilized to accurately determine the number of target molecules. Yet this method relies on the generation of a ‘standard curve’ to quantify...
View ArticleFISH Readout Probes for Colorimetric Detection of SNPs
Single nucleotide polymorphisms (SNPs) result from a single nucleotide mutation in the genome. Many SNPs are closely linked to human diseases and drug efficiency. Single-nucleotide variations (SNVs)...
View ArticleRNA copy number quantification with MERFISH
An improved MERFISH protocol enables the quantification of RNA copy numbers for the characterization of cell-to-cell variability as well as their localizing their spatial position.In 2018, Wang et al....
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Development of a therapeutic peptidomimetic inhibiting main protease to...
The current pandemic is characterized by the intermittent waves of COVID-19 infection. This is due to the high mutation rate of SARS-CoV-2 coronavirus, which is caused, in part, by the low fidelity...
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The DNA i-Motif can form under physiological conditions and is highly...
The DNA i-motif is a DNA structure in which two parallel-stranded duplexes with C.C(+) pairs are intercalated head-to-tail. Multimeric associations or intra-molecular folding can form the i-motif....
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Cytosine Protonation studied in i-Motif oligonucleotides using a Raman Tag
Raman Spectroscopy of cytosines modified with a vibrational tag enables the study of protonated cytosine base pairs in oligonucleotides.A Raman tag is a vibrational tag added to a molecule that allows...
View ArticleShort- and Long-term storage of purified oligonucleotides
How to store DNA and RNA oligonucleotides to enhance your success in all your experiments? Isolated and purified RNA is intrinsically labile. The ubiquity of ribonuclease (RNase) activity further...
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The RNA TrimethylGuanosine or TMG cap
The 2,2,7-trimethylguanosine (TMG or m3G) cap is one of the earliest identified RNA modifications. The TMG-cap ensures nuclear localization of small nuclear RNAs (snRNAs).Research during recent decades...
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Writers, Readers, and Erasers of RNA Modifications
Because of many recent improvements made in the last decades in automated synthesis of biomolecules such as oligonucleotides (DNA/RNA), polypeptides, carbohydrates, and modified derivatives of these,...
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Metastasis Inhibiting Peptides
Peptides inhibit metastasis-associated cancer cell adhesion.Metastasis is a deadly aspect of cancer resulting from interconnected steps, including cell proliferation, developing new blood vessels...
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Thiophosphoramidate Morpholinos, A New Class of Antisense Oligonucleotides
The search is on for better options in antisense technology because significant obstacles still prevent the specific delivery of antisense oligonucleotides (ASOs) into many selected tissues or cells....
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Invader Probes Allow Selective Genomic Target Detection
Invader probes are oligonucleotides or modified oligonucleotides used in invader assays for genotyping of single point mutations (SNPs) and gene expression monitoring. Some invader probes also utilize...
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Circular RNA And Their Biological Functions
What are circular RNAs?Circular RNAs (circRNAs) are classified as non-coding RNAs (ncRNAs). CircRNAs are covalently closed RNA molecules. Initially considered a splicing error, circRNAs appear to have...
View ArticleDelivery Of Active Silencing RNA (siRNA) Into Mitochondria Is Possible
Gao et al., in 2020, reported that transfected siRNAs could enter the mitochondrial matrix and allow the silencing of targeted mitochondrial transcripts. The study investigated whether siRNAs and small...
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Targeting the amino acid tyrosine of peptides or antibodies for selective...
Despite the advances that have been made in molecular medicine, many of the centuries-old diseases continue to persist. These include genetic disorders such as cancer, diabetes, and immunological...
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Pyrene Excimer Probe Based RNA Imaging
Pyrene and its derivatives are polycyclic aromatic hydrocarbons acting as fluorophores. Pyrene-based molecular probes display monomeric fluorescence emission peaks between 375 to 405 nm. The absorption...
View ArticlesiRNA-Conjugates prevent Marburg virus disease
The treatment with mannose and GalNAc conjugated small interfering RNA protects against lethal Marburg virus infection.To prevent Marburg virus disease, Ye et al. recently identified a hexavalent...
View ArticleOne-Step Biotinylation of Carbohydrates
Protein-carbohydrate interactions are essential in numerous biological processes. For the study of protein-carbohydrate interactions, Grün et al., in 2006, reported a simple and fast protocol for the...
View ArticleN-Acetylgalactosamine (GalNAc)-Conjugated Oligonucleotides for Cell Delivery
Biessen & Berkel recently reviewed the 30-year development of oligonucleotide-based therapeutics focusing on the evolution of the GalNAc technology. Basic research investigating substrate...
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