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Analysis and preparation of capped RNAs
Recently emerged technologies now allow the discovery of capped RNAs including NAD-RNA and other small RNA caps. For example, a recently developed method called CapQuant allowed the identification of...
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Keeping track of COVID-19 variants through genomic surveillance and assessing...
Despite the gallant effort by the global scientific community to halt the progression of pandemic caused by COVID-19, the inevitable emergence of novel circulating variants may paint a more realistic...
View ArticleLarge-scale production of capped RNA
Recent improvements in instrumentation and methods now allow the production of capped RNA in large quantities needed to study proteins and protein complexes that bind capped RNAs. Fuchs et al., in...
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Clinical trials assessing the potential to treat heart failure using modified...
Given the negative impact that COVID-19 coronavirus had on the global population, it is hard not to focus on the 'underlying medical condition' as it continues to serve as the major contributing factor...
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Methods for the discovery of cap-like structures
Recently emerged technologies now allow the discovery of capped RNAs, including NAD-RNA and other small RNA caps. For example, a newly developed method called CapQuant allowed the identification of...
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Nicotinamide adenine dinucleotide (NAD) RNA capping and decapping
In eukaryotes, the 5'- terminal ends of cellular mRNAs contain an m7GpppN cap, in which N can be any nucleotide. The RNA helicase eIF4A and the scaffold protein eukaryotic translation initiation factor...
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Development of anti-COVID-19 mRNA vaccine incorporating two proline residues...
For those who closely follow the development of mRNA vaccines targeting COVID-19 coronavirus, one cannot help but notice the artificial insertion of two proline residues to the encoded spike protein....
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Synthesis of branched DNA
Nucleoside phosphoramidite monomers containing two protected hydroxyl functional groups utilized as building blocks allow the synthesis of branched oligonucleotides. During the biosynthesis of...
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The use of phage display technology to develop peptide-based immune...
For a considerable period, immunotherapy employing cytokines such as interleukin-2 or alpha-interferon has been lagging behind other standard treatments, i.e. surgery, chemotherapy and radiotherapy for...
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The tryptophan-cage (Trp-cage) is a stability folded mini protein
The tryptophan-cage or "Trp-cage" is thought to be the smallest fully protein-like folding motif. The Trp-cage mini proteins are small, folded peptides with 18 to 20 residues in length. The 20 residue...
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The siRNA-mediated inhibition of the mTOR complex outperforms rapamycin in...
In the era of COVID-19 coronavirus, we've come to know of the pandemic's taking a heavy toll on the elderly. The older individuals are paying a heavy price as those over 65 y account for the majority...
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Emerging COVID-19 variants prompt Moderna Inc. to generate booster mRNA...
Because of the continuing spread of the COVID-19 coronavirus globally, multiple agencies dedicated to human health including the World Health Organization (WHO) are closely monitoring the evolution and...
View ArticlePhotolysis of caged ATP and caged oligonucleotides
Biological processes are naturally regulated; however, scientists have developed and utilized chemical tools to investigate and control cellular processes. For example, small-molecule probes allow to...
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RNA editing affects the expression, transport, degradation of mRNA: the...
The discovery of the epigenetic modification of DNA has been enlightening as it has opened up novel ways of regulating gene expression in normal processes (ex. embryogenesis) as well as in disease...
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Primers and probes for AIDS/HIV research
Specific DNA probes allow the detection and discrimination of specific nucleic acid target sequences. In the early years of probe development, DNA probe development utilized material from natural...
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How do Oligonucleotide Drugs cross the Blood Brain Barrier?
Like other drugs, oligonucleotide drugs will have to pass through multiple barriers before reaching their cellular targets in the human body. For oligonucleotide-based therapies to work in the central...
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FDA approves 3rd siRNA therapeutic Oxlumo to treat the metabolic disease...
For the oligonucleotide-based genetic medicine, the recent approval of multiple siRNA drugs by Food and Drug Administration marks an important pharmacological shift to molecular biological...
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The incorporation of N1-methyl pseudouridine by T7 bacteriophage derived RNA...
The incorporation of N1-methyl pseudouridine by T7 bacteriophage derived RNA polymerase was instrumental for COVID-19 mRNA vaccine production For the ongoing pandemic, the mRNA vaccines targeting the...
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Microarray analysis using fluor-linked nucleotide probes generated from mRNA...
The advances in molecular biology have enabled a greater understanding of the mechanism underlying cognitive processes and the corresponding disorders. Transmitting the signals by the nervous system...
View ArticleLarge-scale production of capped RNA as a service
During the past decades, multiple laboratories developed messenger RNA (mRNA) vaccines. However, the recent COVID-19 pandemic has made them now a clinical reality. Current events have shown that mRNA...
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