Clik here to view.
Splice switching oligonucleotides
What are splice-switching oligonucleotides (SSOs)?Splice-switching oligonucleotides are short, synthetic, oligonucleotides that act as antisense oligonucleotides. Oligonucleotides containing modified...
View ArticleClik here to view.
Inosine and the RNA World Theory
Kim et al. recently demonstrated that inosine serves as a guanosine substitute. Szostak’s research group tested if 8-oxo-purines could have acted as substrates in primordial RNA to test a new...
View ArticleClik here to view.
Crook siRNA
Crook siRNA is a modified siRNA-DNA construct that can function as a PCR primer. Jiang et al. in 2005 reported that the "Crook" siRNA-DNA construct functions as a primer for PCR and when transfected...
View ArticleClik here to view.
Humans take their microbes into space
A recent study sampled the microbe diversity that can survive in the International Space Station (ISS). The study revealed that humans take their microbes with them into space. Molecular and...
View ArticleClik here to view.
L-DNA, the mirror-image form of d-DNA
L-DNA is a mirror-image form of the naturally occurring d-conformation of DNA. L-DNA forms a left-helical double-helix but has similar physical characteristics in regards to solubility, duplex...
View ArticleClik here to view.
Genotypes, Phenotypes and Mutagenesis
In cells, DNA damage leads to heritable mutations that can cause and accelerate disease progression and drug resistance in pathogenic bacteria as well as in cancers. However, all cells possess...
View ArticleClik here to view.
A PEPTIDE BASED ZINC PROBE
Fluorescent probes used as biological indicators can provide useful information about physiological changes in cells. Rationally designed probes containing dyes that respond linearly to the target...
View ArticleClik here to view.
A Molecular Beacon for Cancer Point-of-Care
A Molecular Beacon for Cancer Point-of-Care Diagnostics“Point-of-Care” can positively impact health care delivery as well as address challenges of health disparities. What is “Point-of-Care”? In the...
View ArticleClik here to view.
Crosslinking Ligation And Sequencing of Hybrids or CLASH
A technique called CLASH as described in 2014 by Helwak and Tollervey allows high-throughput identification of RNA-RNA interaction sites without bioinformatic predictions. Next-generation deep...
View ArticleClik here to view.
mRNA Capping and Decapping Enzymes
Capping and decapping of mRNA are cellular events during gene expression. The chemical nature of the 5′ end of RNA appears to determine the stability of RNA during RNA processing, and localization, as...
View ArticleClik here to view.
Efficient intracellular delivery of prodrugs with ProTides
ProTides are prodrugs allowing the efficient intracellular delivery of nucleoside analog monophosphates and monophosphonates. In ProTides the hydroxyls of the monophosphate or monophosphonate groups...
View ArticleBio-Synthesis, Inc. to supply peptide-BNA for the treatment of triple...
Bio-Synthesis, Inc. to supply peptide-BNA for the treatment of triple negative breast cancer Cancer continues to pose a problem as approximately ~1500 are expected to die daily from cancer in the U.S....
View ArticleA clinical study demonstrates the feasibility of RNA interference therapy...
A clinical study demonstrates the feasibility of RNA interference therapy targeting ribonucleotide reductase in melanoma patients Melanoma continues to be the focus of an intensive investigation...
View ArticleClik here to view.
BNA miRNA Probes for the Study of Osteoarthritis
BNA enhanced microRNA (miRNA) probes are powerful tools for the development of highly specific in situ hybridization (ISH) assays allowing studying miRNA expression levels in cellular tissues. In...
View ArticleClik here to view.
Chemical synthesis of artificial nucleotides and their incorporation by...
Chemical synthesis of artificial nucleotides and their incorporation by modified enzymes: BNA-oligonucleotides for therapyThe naturally occurring DNA or RNA possesses multiple vulnerabilities as...
View ArticleClik here to view.
The utility of 6-thio-2’-deoxyguanosine for novel telomerase-dependent...
The utility of 6-thio-2’-deoxyguanosine for novel telomerase-dependent anti-cancer therapy Unlike prokaryotic genomes consisting of circular DNA, the eukaryotic genomes consist of linear DNA, which is...
View ArticleClik here to view.
Genome editing: modified CRISPR/Cas9 corrects genetic mutation causing...
Genome editing: modified CRISPR/Cas9 corrects genetic mutation causing Alzheimer’s disease or cancer without cleaving DNAGenetic mutations account for a significant fraction of human disorders....
View ArticleClik here to view.
Phase II clinical trial of Hedgehog pathway inhibitor with low-dose...
Phase II clinical trial of Hedgehog pathway inhibitor with low-dose cytarabine (Ara-C) extends the survival of acute myeloid leukemia (AML) patientsAcute myeloid leukemia (AML) accounts for >140,000...
View ArticleClik here to view.
Bio-Synthesis, Inc. supplied modified oligonucleotide identified the strand...
Bio-Synthesis, Inc. supplied modified oligonucleotide identified the strand tracked by CMG complexes to unwind double helixto form bi-directional forks for DNA replicationDNA replication, which is...
View ArticleClik here to view.
Spermine-modified cationic ZNA oligonucleotide for in situ hybridization,...
For hybridization, the rate of renaturation is thought to be proportional to the square root of the length of the complementary single strands (Niranjani et al., 2016). In practice, shorter...
View Article