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Identifying RNAs with RNA tags
Natural RNA tags added to RNA of interest allow detection, characterization, labeling and affinity purification of RNA target molecules. For example, small aptamer RNAs that bind with high affinity and...
View ArticleMethods for the study of RNA and RNA-protein complexes
Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. The reasons are as follows.• RNA...
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Enhancer RNAs, a new class of RNAs
The development of next-generation sequencing (NGS) methods revolutionized the study of genomic transcriptionally active regions. NGS now makes it possible to catalog transcripts from coding sequences...
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BNAs dramatically improve CRISPR Cas9 specificity
BNAs dramatically improve the specificity of CRISPR-Cas9. The addition of BNAs (2′,4′-BNANC[N-Me]) to CRISPR-RNAs (crRNAs) dramatically improves the accuracy in CRISPR-based gene-editing. In this...
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Horseradish Peroxidase for Probe Design and Conjugation
Horseradish Peroxidase, or HRP, allows the design and synthesis of molecular probes such as in situ hybridization probes to increase signal amplification. HRP, when conjugated to hybridization probes,...
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Conjugation of Taxol or Paclitaxel to DNA nanoparticles
To increase solubility and effectiveness of Paclitaxel, the conjugation of the drug to gold nanoparticles via DNA linkers is possible. According to Zhang et al., resulting conjugates usually show a...
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Synthesis of Paclitaxel-Fluorescein-DNA conjugates
To overcome disadvantages of drugs with poor water solubility different strategies such as the conjugation of the drug to small molecules, carbohydrates, oligonucleotides, peptides, or other molecules...
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Methylene Blue Conjugates
The last two decades have seen an intensified development of nanoscale calorimetric and electrochemical sensors. Some of these sensors utilize on–target-inducible folding or unfolding of...
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Minor Groove Binder Phosphoramidites
Minor groove binder (MGB) phosphoramidites are new tools that allow adding the MGB moiety dihydropyrroloindole-carboxylate (CDPI3) to the 3’- or 5’-end of oligonucleotides. MGB phosphoramidites can be...
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Identifying RNAs with RNA tags
Natural RNA tags added to RNA of interest allow detection, characterization, labeling and affinity purification of RNA target molecules. For example, small aptamer RNAs that bind with high affinity and...
View ArticleMethods for the study of RNA and RNA-protein complexes
Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. The reasons are as follows.• RNA...
View ArticleClik here to view.
Enhancer RNAs, a new class of RNAs
The development of next-generation sequencing (NGS) methods revolutionized the study of genomic transcriptionally active regions. NGS now makes it possible to catalog transcripts from coding sequences...
View ArticleClik here to view.
BNAs dramatically improve CRISPR Cas9 specificity
BNAs dramatically improve the specificity of CRISPR-Cas9. The addition of BNAs (2′,4′-BNANC[N-Me]) to CRISPR-RNAs (crRNAs) dramatically improves the accuracy in CRISPR-based gene-editing. In this...
View ArticleClik here to view.
Horseradish Peroxidase for Probe Design and Conjugation
Horseradish Peroxidase, or HRP, allows the design and synthesis of molecular probes such as in situ hybridization probes to increase signal amplification. HRP, when conjugated to hybridization probes,...
View ArticleClik here to view.
Conjugation of Taxol or Paclitaxel to DNA nanoparticles
To increase solubility and effectiveness of Paclitaxel, the conjugation of the drug to gold nanoparticles via DNA linkers is possible. According to Zhang et al., resulting conjugates usually show a...
View ArticleClik here to view.
Synthesis of Paclitaxel-Fluorescein-DNA conjugates
To overcome disadvantages of drugs with poor water solubility different strategies such as the conjugation of the drug to small molecules, carbohydrates, oligonucleotides, peptides, or other molecules...
View ArticleClik here to view.
Methylene Blue Conjugates
The last two decades have seen an intensified development of nanoscale calorimetric and electrochemical sensors. Some of these sensors utilize on–target-inducible folding or unfolding of...
View ArticleClik here to view.
Pseudouridine, an abundant post-transcriptional RNA modification
Pseudouridine (), one of the most abundant modified RNA molecules, is synthesized in situ by synthases. Naturally occurring RNA molecules are often modified. Over 100 different types of RNA...
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Ultrafast Oligonucleotide Photo Cross Linking
The nucleoside analogue 3-cyanovinyl carbazole, CNVK, enables ultrafast reversible photo-cross-linking of oligonucleotides. Recently a new non-enzymatic and non-invasive technologies for nucleic acid...
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Applications of Photocleavable Oligonucleotides
Photocleavable oligonucleotides have many applications in biological and medicinal research. Photoactive groups can function as protecting groups of oligonucleotides and for a variety of other...
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