| Spectra of available Fluorescent Dyes | |||||||||
| Fluorescent Dye | Abs | Em | Labelling Position | Purification Option | Alternative to | ||||
| 5' | Int | 3' | HPSF | HPLC | PAGE | ||||
| Alexa Fluor 350 [Alexa350] | 346 | 442 | x | x | x | x | AMCA, DyLight350, iFluor350, CF350, | ||
| ATTO 390 | 390 | 476 | x | x | x | x | |||
| Dy-415 [DY415] | 418 | 467 | x | x | x | x | Coumarin (DEAC) | ||
| ATTO 425 [ATTO425] | 436 | 484 | x | x | x | x | Alexa Fluor 425 | ||
| ATTO 465 [ATTO465] | 453 | 508 | x | x | x | x | LC 480 Cyan 500 | ||
| Bodipy FL [BOFL] | 504 | 510 | x | x | x | x | |||
| Alexa Fluor 488 [Alexa488] | 495 | 519 | x | x | x | x | CY2 DY-495ATTO 495 | ||
| FAM [FAM] | 495 | 520 | x | x | x | x | CY2 DY-495ATTO 495 | ||
| Fluorescein isothiocyanate [FITC] | 495 | 520 | x | x | x | x | CY2 DY-495ATTO 495 | ||
| Fluorescein [FLU] | 495 | 520 | x | x | DY-495 ATTO 495 | ||||
| Fluorescein-dT [FLUdT] | 494 | 522 | x | x | DY-495 ATTO 495 | ||||
| ATTO 488 [ATTO488] | 501 | 523 | x | x | x | x | Cy2 | ||
| Oregon Green 488 [OG488] | 496 | 524 | x | x | x | x | Cy2 | ||
| Oregon Green 514 [OG514] | 506 | 526 | x | x | x | x | Cy2 | ||
| Rhodamine Green [RGR] | 503 | 528 | x | x | x | x | Cy2 | ||
| TET [TET] | 521 | 536 | x | x | x | x | CAL Fluor Gold 540 | ||
| ATTO 520 [ATTO520] | 516 | 538 | x | x | x | x | CAL Fluor Gold 540 Rhodamine 6G | ||
| JOE [JOE] | 520 | 548 | x | x | x | x | CAL Fluor Gold 540 Rhodamine 6G | ||
| Yakima Yellow [YAKYE] | 530 | 549 | x | x | VICCAL Fluor 560 | ||||
| Bodipy 530/550 [BO530] | 534 | 551 | x | x | x | x | VICCAL Fluor 560 | ||
| HEX [HEX] | 535 | 556 | x | x | x | x | VICCAL Fluor 560 SIMA | ||
| Alexa Fluor 555 [Alexa555] | 555 | 565 | x | x | x | x | Cy3TRITC & TMRAlexa Fluor 546 | ||
| Dy-549 [DY549] | 553 | 566 | x | x | x | x | Quasar 570 Dragonfly Orange | ||
| Bodipy TMR-X [BOTMRX] | 544 | 570 | x | x | x | x | Quasar 570 Dragonfly Orange | ||
| Cyanine3 [CY3] | 552 | 570 | x | x | x | Quasar 570 Dragonfly OrangeTYE 563 | |||
| ATTO 550 [ATTO550] | 554 | 576 | x | x | x | x | NEDCy3Rhodamine B & 6G | ||
| TAMRA [TAM] | 544 | 576 | x | x | x | Rhodamine 6G Rhodamine B | |||
| Rhodamine Red [RRE] | 560 | 580 | x | x | x | x | Rhodamine 6G Rhodamine B | ||
| ATTO 565 [ATTO565] | 563 | 592 | x | x | x | x | PETAlexa Fluor 594 | ||
| Fluorescent Dye | Abs | Em | Labelling Position | Purification Option | Alternative to | ||||
| 5' | Int | 3' | HPSF | HPLC | PAGE | ||||
| ROX [ROX] | 575 | 602 | x | x | x | x | Texas Red Alexa Fluor 594 | ||
| Texas Red [TxRed] | 583 | 603 | x | x | x | x | ROX™Alexa Fluor 594 | ||
| Cyanine3.5 [CY35] | 588 | 604 | x | x | Alexa Fluor 594 CAL Fluor Red 610 | ||||
| LightCycler 610 [LC610] | 590 | 610 | x | x | x | x | Alexa Fluor 594 CAL Fluor Red 610 | ||
| ATTO 594 [ATTO594] | 601 | 627 | x | x | x | x | Alexa Fluor 594 CAL Fluor Red 610 | ||
| DY-480 XL [DY480] | 500 | 630 | x | x | x | x | |||
| DY-610 [DY610] | 610 | 630 | x | x | x | x | Alexa Fluor 610 | ||
| ATTO 610 [ATTO610] | 615 | 634 | x | x | x | x | Alexa Fluor 610 | ||
| LightCycler 640 [LC640] | 625 | 640 | x | x | x | x | |||
| Bodipy 630/650 [BO630] | 625 | 640 | x | x | x | x | Alexa Fluor 633 | ||
| ATTO 633 [ATTO633] | 629 | 657 | x | x | x | x | LIZAlexa Fluor 633 | ||
| Alexa Fluor 647 [Alexa647] | 650 | 665 | x | x | x | x | Cy5 Quasar 670 | ||
| Bodipy 650/665 [BO650] | 650 | 665 | x | x | x | x | Cy5 Quasar 670 | ||
| ATTO 647N [ATTO647N] | 644 | 669 | x | x | x | x | Cy5 Quasar 670 | ||
| Cyanine5 [CY5] | 649 | 670 | x | x | x | Quasar 670TYE 655 | |||
| DY-649 [DY649] | 655 | 674 | x | x | x | x | Quasar 670 | ||
| Cyanine5.5 [CY55] | 675 | 694 | x | x | Alexa Fluor 680TYE 705 | ||||
| ATTO 680 [ATTO680] | 680 | 700 | x | x | x | x | Alexa Fluor 680Alexa Fluor 700 | ||
| DY-682 [DY682] | 690 | 709 | x | x | x | x | IRDye 700Quasar 705 | ||
| ATTO 700 [ATTO700] | 700 | 719 | x | x | x | x | IRDye 700Quasar 705 | ||
| ATTO 740 [ATTO740] | 740 | 764 | x | x | x | x | Cy7Alexa Fluor 740 | ||
| DY-782 [DY782] | 782 | 800 | x | x | x | x | IRDye 800 | ||
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Spectra of available Fluorescent Dyes
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