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ID | Label 5’ | Oligonucleotide Sequence (5’>3’) | Label 3’ | |
|
|
| ||
|
| Nucleoprotein – N Protein |
| |
2019-nCoV_N1-F |
| GACCCCAAAATCAGCGAAAT |
| |
2019-nCoV_N1-R |
| TCTGGTTACTGCCAGTTGAATCTG |
| |
2019-nCoV_N1-P | FAM | ACCCCGCATTACGTTTGGTGGACC | BHQ1 | |
2019-nCoV_N2-F |
| TTACAAACATTGGCCGCAAA |
| |
2019-nCoV_N2-R |
| GCGCGACATTCCGAAGAA |
| |
2019-nCoV_N2-P | FAM | ACAATTTGCCCCCAGCGCTTCAG | BHQ1 | |
2019-nCoV_N3-F |
| GGGAGCCTTGAATACACCAAAA |
| |
2019-nCoV_N3-R |
| TGTAGCACGATTGCAGCATTG |
| |
2019-nCoV_N3-P | FAM | AYCACATTGGCACCCGCAATCCTG | BHQ1 | |
|
|
| ||
| Nucleoprotein – N Protein |
| ||
HKU-NF |
| TAATCAGACAAGGAACTGATTA |
| |
HKU-NR |
| CGAAGGTGTGACTTCCATG |
| |
HKU-NP | FAM | GCAAATTGTGCAATTTGCGG | TAMRA | |
|
|
| |
| Nucleoprotein - N Protein |
| |
2019-nCoV-NFP |
| GGGGAACTTCTCCTGCTAGAAT |
|
2019-nCoV-NRP |
| CAGACATTTTGCTCTCAAGCTG |
|
2019-nCoV-NP | FAM | TTGCTGCTGCTTGACAGATT | TAMRA |
| ORF1ab |
| |
2019-nCoV-OFP |
| CCCTGTGGGTTTTACACTTAA |
|
2019-nCoV-ORP |
| ACGATTGTGCATCAGCTGA |
|
2019-nCoV-OP | FAM | CCGTCTGCGGTATGTGGAAAGGTTATGG | BBQ1 |
|
| . | |
| ORF1b-nsp14 |
| |
HKU-ORF1b-nsp14F |
| TGGGGYTTTACRGGTAACCT |
|
HKU- ORF1b-nsp14R |
| AACRCGCTTAACAAAGCACTC |
|
HKU-ORF1b-nsp141P | FAM | TAGTTGTGATGCWATCATGACTAG | TAMRA |
ID | Label 5’ | Oligonucleotide Sequence (5’>3’) | Label 3’ |
|
| CDC |
|
|
| RNAse Protein |
|
RP-F |
| AGATTTGGACCTGCGAGCG |
|
RP-R |
| GAGCGGCTGTCTCCACAAGT |
|
RP-P RNAse P | FAM | TTCTGACCTGAAGGCTCTGCGCG | BHQ-1 |
Others
| ORF1b-nsp14 |
| |
HKU-ORF1b-nsp14F |
| TGGGGYTTTACRGGTAACCT |
|
HKU- ORF1b-nsp14R |
| AACRCGCTTAACAAAGCACTC |
|
HKU-ORF1b-nsp141P | FAM | TAGTTGTGATGCWATCATGACTAG | TAMRA |
References
CDC - US Centers for Disease Control and Prevention. (2020, February 4). Real-time RT-PCR panel for detection 2019-novel coronavirus.
Charite Virology - Corman et al. (2020, January 17). Diagnostic detection of 2019-nCoV by real-time RT-PCR.
CDC China - National Institute For Viral Disease Control and Prevention. (2020, January 21). Specific primers and probes for detection 2019 novel coronavirus.
HKU MedProtocol
World Health Organization (WHO)
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