BNA FISH Probe for Chromosomal DNA Detection

Even though fluorescence in situ hybridization - FISH probe has been widely used in cytogenetic applications, it is generally time-consuming and lack of sensitivity and resolution. Bio-Synthesis's high affinity BNA enchanced FISH Probes (BFISH) provides an alternative solution for a fast, sensitive and specific detection of chromsomal DNA.

You design. We can Help

We are happy in assist you with BNA FISH probes. Please contact us for more information. YOu may also design your own BNA FISH probes using guideline below.
 

•  Detection probes are typically 20-25 nucleotides in length. However, shorter or longer probes can also be used.
• Avoid stretches of 3 or more Gs or Cs. Avoid stretches of more than 4 BNA bases, except when very short (9-10 nt) oligonucleotides are designed.
• Avoid BNA with self-complementarity since BNA hybridizes very tightly to other BNA residues.
• Keep the GC-content between 30-60 %.
• A Tm of approximately 75 °C is recommended.
• No BNA bases should be placed in palindromes (G-C base pairs are more critical than A-T base pairs).

Other useful links:

1. Can you suggest a BNA experimental condition for FISH application?
2. Do you have suggested buffers for BNA experiment?
3. Bridged Nculeic acid Design Guidelines
4. BNA for Duplex and Triplex Formation
5. Allele Specific BNA Primer Design