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  • 02/23/14--00:00: Antiviral Peptides
  • Many peptides have been reported to prevent viral attacks

    Wouldn’t it be great if foods or drinks enabled us humans to prevent viral or bacterial infections? Or, if nasal or oral spray containing antiviral compounds prevented the infection of us humans by viruses. Imagine that there would be no flu, no colds, no AIDS or any other disease caused by a virus anymore and that the occurrence of these diseases is prevented by just eating or drinking one of your favorite foods or drinks. Surprisingly, some major food products, such as milk and milk based products, reported to be heavily consumed in some western, North African and other countries have been found to contain antiviral substances. When investigate some active components turned out to be proteins and peptides derived from the proteins present in the milk of mammals and humans. Especially some proteins and peptides found in adult milk or colostrum, the mother’s milk produced within the first 24 hours after birth, have been reported to bind to both virus particles and cellular receptors to prevent viral adsorption and entry, or to interfere with intracellular replication events or the synthesis of secondary viral components. Apparently milk, cheeses and yoghurt are foods that can help preventing viral and bacterial infections. Milk and cheese, both have been shown to contain proteins and peptides that have antiviral and antibacterial properties. And over the years many peptides have been found to prevent virus attacks or the entry of a virus into human cells. These types of peptides are called antiviral peptides.

    H1N1 Flu Virus
    H1N1 Flu Virus

    Antiviral peptides (AVP) are peptides that have been experimentally verified to block virus attachment or the entry of a virus into host cells. It may just be possible that these types of peptides interfere with key steps a pathogenic mammalian and human virus needs to enter a cell. Scientists now hope that these inhibitory peptides could be used in the future as a starting point for the design of more active molecules for targeting of crucial molecules a virus needs to infect a host. The potential targets maybe carbohydrates, lipids, proteins, glycoproteins and oligonucleotides, involved in virus attachment, fusion and replication, and possibly other molecular functions yet to be determined. If this approach turns out to be successful antiviral peptides may become the preferred choice for the production of vaccines or therapeutics in the near future because of their relatively low molecular weight, lower toxicity, their rapid elimination from the host, possibly lesser side effects and also due to a lower cost if cost effective and efficient synthesis strategies are used.
     
    A list of 144 antiviral peptides can be found in the “Antimicrobial Peptide Database (APD2)” [http://aps.unmc.edu/AP/main.php]. Peptides such as lactoferricin B, defensines, magainines, mellitins that have been experimentally verified to exhibit both antiviral and antibacterial activities and many others can be found in this database. Apparently, many of these peptides exhibit diverse activities including inhibition activities against gram positive and gram negative bacteria, viruses, fungi, some parasites, HIV, and even some mammalian cells or cancer cells. Many of these peptides originate from insects, frogs and mammals but may not be limited to just these species. For example, the casein proteins, whey proteins and their derived peptides found in milk all have antiviral activities and function as antiviral and immune regulatory factors by regulating the innate immune response. It has been found that the regulation is achieved by, both, up-regulation of the response to enhance the killing of viruses, and down-regulation to reduce detrimental conditions that may damage the host, for example, through sepsis, a potentially fatal whole-body inflammation caused by a severe infection.
     
    In addition, VanCompernolle and others reported in 2005 that amphibian skin is a rich source of broad-spectrum antimicrobial peptides including some that haveantiviral activity. The research group identified three peptides called caerin 1.1, caerin 1.9, and maculatin 1.1 that completely inhibited HIV infection of T cells within minutes of exposure to the virus at concentrations that were not toxic to target cells. The researchers were also able to show that these peptides also suppressed infection by the murine leukemia virus but not by the reovirus, a structurally unrelated nonenveloped virus, and that preincubation using these peptides prevented viral fusion to target cells and disrupted the HIV envelope. The researchers could show that these amphibian peptides also were highly effective in inhibiting the transfer of HIV by dendritic cells to T cells. The scientists than reasoned that amphibian-derived peptides can access DC-sequestered HIV and destroy the virus before it can be transferred to T cells making these peptides promising tools that could function as topical inhibitors of mucosal HIV transmission.
     
    Thakur et al. in 2012 reported in the Journal of Nucleic Acids Research a collection of 1245 peptides which were experimentally verified to have antiviral activity. The scientists reported that these peptides target important human viruses such as the influenza viruses, a RNA virus that causes the flu, the human immunodeficiency virus (HIV), a lentivirus that causes the acquired immunodeficiency syndrome (AIDS), ultimately resulting in the progressive failure of the immune system which than allows life-threatening opportunistic infections and cancers to develop in patients, the hepatitis C virus (HCV), a small, enveloped, positive-sense single-stranded RNA virus, that causes hepatitis C in humans, and the virus that causes the severe acute respiratory syndrome, a viral respiratory disease of zoonotic origin caused by the SARS coronavirus (SARS-CoV) (SARS), and multitude of other viruses. The research group reported that, after removing redundant peptides, they selected 1056 peptides which they further divided into 951 training and 105 validation data sets. Using features such as various peptides sequences, i.e. motifs and alignments followed by amino acid composition and physicochemical properties of the known antiviral peptides a bioinformatic approach called a “Support Vector Machine” approach was developed by this research group allowing the development and design of a prediction method algorithm for the identification of new antiviral peptides based on their amino acid sequences. The newly developed algorithm called AVPpred is a web server that allows the prediction of highly effective antiviral peptides that maybe helpful to researchers working on peptide-based antiviral development. The web server is freely available at http://crdd.osdd.net/servers/avppred.
     
    The design of peptide based viral inhibitors is an example of the potential use of synthetic peptides to prevent viral infections. Miller at al. in 1989 described the early design of such a peptide inhibitor that was used to study its inhibitory effect on HIV-1 protease. The research group reported the structure of synthetic HIV-1 protease in complex with a substrate-based peptide inhibitor at a resolution of 2.3 Å. The hexa-peptide inhibitor containing the amino acid sequence N-acetyl-Thr-Ile-Nle-Ψ[CH2-NH]–Nle-Gln-Arg-amide was shown to bind to the active site of the protease in an extended conformation. The reasoning behind structural based studies is that the understanding of the details of inhibitor-enzyme or inhibitor-viral protein interactions will be useful for the design of potential efficient and effective antiviral or antibacterial peptides.

    In summary, antiviral peptides may offer hope for the design of better and less expensive therapeutics to help prevent viral infections.


    Reference

    Miller M, Schneider J, Sathyanarayana BK, Toth MV, Marshall GR, Clawson L, Selk L, Kent SB, Wlodawer A.; Structure of Complex of Synthetic HIV-1 Protease with a Substrate-Based Inhibitor at 2.3 A Resolution. Science (1989) 246 p.1149
     
    Nishant Thakur, Abid Qureshi, and Manoj Kumar; AVPpred: collection and prediction of highly effective antiviral peptides. Nucleic Acids Res. 2012 July; 40 (Web Server issue): W199–W204. Published online 2012 May 24. doi:  10.1093/nar/gks450, PMCID: PMC3394244.
     
    Scott E. VanCompernolle, R. Jeffery Taylor, Kyra Oswald-Richter, Jiyang Jiang, Bryan E. Youree, John H. Bowie, Michael J. Tyler,  J. Michael Conlon, David Wade, Christopher Aiken, Terence S. Dermody, Vineet N. KewalRamani, Louise A. Rollins-Smith, and Derya Unutmaz;  Antimicrobial Peptides from Amphibian Skin Potently Inhibit Human Immunodeficiency Virus Infection and Transfer of Virus from Dendritic Cells to T Cells.  J. Virol. September 2005 vol. 79 no. 18 11598-11606.
     
     
     

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    The Chemical Synthesis of Oligonucleotides

    By Andrei Laikhter and Klaus D. Linse

    The study of nucleic acids has now become a fruitful and dynamic scientific enterprise. Nucleic acids are of unique importance in biological systems because genes are the fundamental unit of heredity. The process how genes are expressed in all living organisms is fundamentally important and most genes are located in the chromosomes within the cell nucleus. Genes express themselves via a protein machinery in the cytoplasm. The genetic material was identified as deoxyribonucleic acid (DNA) in 1944 (Avery et al. 1944). Next, the double-helical nature of DNA was revealed in 1953 by Francis Crick, James Watson and Maurice Wilkins. Furthermore, the combined action of multiple genes defines the properties or phenotype of higher organisms. Even to this date multi-gene characteristics are difficult to analyze. Genes are made up of deoxyribonucleic acid or DNA, and each gene is a linear segment, or polymer, of a long DNA molecule. However, genes have been found to be nonrandomly distributed on the chromosomes and vary enormously in size and intron-exon structure. A DNA polymer, or DNA oligonucleotide, contains a linear arrangement of subunits called nucleotides. There are four types of nucleotides. Each nucleotide has three components; a phosphate group, a sugar and a base that contains nitrogen within its structure. The sugar moiety in DNA oligonucleotides is always dexoyribose, and there are four alternative bases: adenine (Ade, A), thymine (Thy, T), guanine (Gua, G), and cytosine (Cyt, C). The phosphate groups and the deoxyribose sugars form the backbone of each DNA stand. The bases are joined to the deoxyribose sugar and stick out to the side. Both, DNA and ribonucleic acid (RNA), consist of 5’-3’ phosphodiester-linked nucleotide units that are composed of a 2’-deoxy-D-ribose (DNA) or D-ribose (RNA) in their furanose forms and a heteroaromatic nucleobase (A, T, G, and C; A, U, G, C), and the resulting oligonucleotide chain is composed of a polar, negatively charged sugar-phosphate backbone and an array of hydrophobic nucleobases. The amphiphilic nature of these polymers dictates the assembly and maintenance of secondary and tertiary structures the oligonucleotides can form. In double stranded DNA, the bases of one strand are paired with the bases in the other strand. Adenine (A) in one strand is paired with thymine (T) in the other strand and guanine (G) in one strand is paired with cytosine (C) in the other strand as well. In the DNA duplex structure, genetic information is stored as a linear nucleotide code. This code can be accessed and replicated. RNA, or ribonucleic acid, is another structurally related essential biopolymer. RNA differs from DNA in having the sugar ribose in place of the deoxyribose. Furthermore, in RNA the thymine (T) is replaced with uracil (U). The bases A and G are purine bases as they contain a double ring structure called the purine ring. The other two bases, C and T, are pyrimidine bases because they contain a single pyrimidine ring. Hydrogen bonds hold the two strands together.

    SChemical Synthesis of Oligonucleotides

    Structures of nucleic acids

    Figure 1: Structures of nucleic acids.

    Figure 2: Atomic numbering and definitions of torsion angles for one nucleotide according to the UPAC nomenclature. The designation of chain direction and main chain atoms of i th unit in a polynucleotide chain and the atom numbering for the bases of common nucleosides and nucleotides are illustrated. Hydrogen atoms carry the same numbers as the heavy atoms to which they are attached. The name in parenthesis applies when the 'd' in parenthesis in the formula is present.

    (Source: www.chem.qmul.ac.uk/iupac/misc/pnuc1.html).

    Representative types of base pairs.

    Figure 3: Representative types of base pairs. Purines and pyrimidines can form base pairs through hydrogen bonds. Watson-Crick, Hoogsten and wobble base pairs are the most common. An extensive list of graphical base pairs can be found in “Wolfram Sanger: Principles of Nucleic-Acid Structure. Spinger-Verlag New York Berlin Heidelberg Tokyo, pp 120.”

    In biological systems, nucleic acids exist in higher ordered structures held together through self-assembly. Since hydrogen bonding and base stacking are the major driving force for this self-assembly the structural properties of nucleotides can affect the self-assembly process. These molecular forces dictate the final tertiary structure of nucleic acids (The RNA World).

    The central dogma of molecular biology, originally described by Francis Crick, illustrates how DNA is transcribed into RNA which is ultimately translated into protein sequences. According to the dogma nucleic acid alone may store information or specify the sequence of gene products, proteins. Proteins are never able to specify a nucleic acid or protein sequence.

    The central dogma of molecular biology

    Figure 4: The central dogma of molecular biology.

    Structures of oligonucleotides

    Figure 5: Structures of oligonucleotides.

    In the beginning years, the chemical synthesis of specific oligoribo- and oligodeoxyribonuclotides has been regarded as a somewhat esoteric chemistry of natural compounds. The advent of gene technology and the development of new chemical and analytical methods such as high performance liquid chromatography (HPLC), 31P-NMR spectroscopy and automated synthesis methods has changed this all. The development and introduction of commercially available “gene synthesizing machines” following the development of chemical DNA and RNA synthesis strategies has made the synthesis of natural and artificial oligo-nucleotides a routine procedure as well as more cost-efficient and faster.

    To allow for a successful oligonucleotide synthesis the following fundamental prerequisites need to be established:

    • All reagents should be soluble in non-aqueous solvents.
    • The amino and hydroxyl functions of nucleotide bases and sugar residues must be suitably blocked.
    • Protecting groups, introduced during synthesis, must be stable under conditions of chain elongation when the internucleotide phosphodiester bonds are formed.
    • Protecting groups should be labile enough to allow removal at the end of the synthesis without damaging the reaction products.
    • Final yields of the synthesis should be almost quantitative to avoid products with failure sequences.

    Several strategies have been developed in the past which comply with these limiting provisions. The phosphodiester and the phosphotriester approaches, both, utilize protection of the 3’ and 5’ hydroxyl groups of the deoxyribose. The phosphotriester uses a third protecting group for the protection of the internucleotide bond. A third approach called the “phosphate” procedure employs compounds with trivalent phosphorus and can be regarded as a trimester method. The development of oligonucleotide synthesis started with the phosophotriester method in 1955 but the fist significant success is reported to be achieved using the phosphodiester method when the genes for alanine and tyrosine suppressor tRNAs of yeast and E. coli were synthesized in the 1980s.

    Figure 6: Outline of the synthesis of the dinucleotide d(TpT) by Michelson and Todd reported in 1955. In this approach, 3’-O-acetylthymidine was phosphorylated with a phosphorochloridate, and the phosphate group was protected with a benzyl group. The structure of the dinucleotide was confirmed by enzymatic digestion (Michelson & Todd, 1955). This synthesis approach later became known as the phosphotriester approach.

    The literature reports that Gobind Khorana, in 1956, accidentally discovered the phosphodiester method for the chemical synthesis of deoxyribo-oligonucleotides (Khorana et al., 1956). The exploitation and further development of this method by many scientists in subsequent years for the chemical synthesis of deoxyribo-oligonucleotides led to the elucitation of the genetic code and the first total synthesis of a gene (Khorana et al., 1956; Khorana, 1961, 1969, 1979). Marshall Nirenberg and Gobind Khorana broke the genetic code and could assign code words called codons. Codons are triplets of nucleotides coding for the twenty amino acids. Both scientists received the Nobel Prize in Physiology or Medicine in 1968 together with Robert Holley.

    Phosphotriester Method

    Where B = A,C, G or T nucleo bases; R1, R2, R3 = protecting groups; X = Halogen

    Figure 7: General outline of the phosphotriester method.

    Figure 8: Outline of Khorana’s synthesis approach later known as the phosphodiester approach. In this approach, 5’-O-tritylthymidine and 3’-O-acetylthymidine 5’-phosphate are reacted in the presence of toluene-4-sulfonyl chloride (TsCl) or N1,N3-dicyclohexylcarbodiimide (DCC) in a pyridine solution. The removal of the trityl and acetyl group yields the d(TpT) dinucleotide (Khorana et al., 1956; 1957; Gilman and Khorana, 1958).

    Posphodiester Method
    phosphodiester method

    Where B = A,C, G or T nucleo bases; R1, R2 = protecting groups

    Figure 9: General outline of the phosphodiester method.

    In the phosphodiester method the phosphate group between the two nucleotides is unprotected which makes the resulting compounds only soluble in organic solvents to a limited extent.

    Phosphite Method
    Phosphite Method

    Where B = A,C, G or T nucleo bases; R1, R2, R3 = protecting groups; X = Halogen, N(CH3)2, morpholine

    Figure 10: Chemistry of phosphite method.

    Willi Bannwarth in 1985 reported a simple synthesis of phosphoramidite dinucleotides with two different phosphorous-protecting groups for the synthesis of 2′-oligodeoxynucleotides on a polymer support called the “Phosphite-Triester Method.

    Even though oligonucleotides can be assembled manually in a step-wise fashion this process has been and is quite laborious and demanding. The availability of commercial DNA synthesizers has made the process easier and more cost effective. The principle of solid phase synthesis was first developed and applied to the synthesis of polypeptides by Robert Bruce Merrifield (July 15, 1921 – May 14, 2006), an American biochemist who won the Nobel Prize in Chemistry in 1984 for the invention of solid phase peptide synthesis. He realized that the key to a successful synthesis is to anchor the first monomer to an insoluble polymeric support. Other monomers can then be joined, one by one, to the fixed terminal end of the growing polymer. At the end of the synthesis, the completed polymer chain can be detached from the insoluble polymer and purified. This process has been further optimized over the years to become highly efficient and has now become a fundamentally important method employed in automated oligonucleotide synthesizers.

    I. General Methods of Solid Phase Oligonucleotide Synthesis.

    a) Phosphoramidite method

    The phosphoramidite method of DNA synthesis is currently considered as the standard synthesis method used in most automated synthesizers today. This method allows achieving the high coupling efficiencies needed to synthesize longer and longer oligonucleotides with low amounts of failure sequences. The oligonucleotide phosphoramidite synthesis chemistry was introduced nearly 20 years ago (McBride and Caruthers, 1983). Building blocks used for synthesis are commonly referred to as “monomers”, which are activated DNA nucleosides (phosphoramidites). The dimethoxytrityl (DMT) group is used to protect the 5’-end of the nucleoside, a β-cyanoethyl group protects the 3’-phosphite moiety, and may also include additional groups that serve to protect reactive primary amines in the heterocyclic nucleo bases. The protecting groups are selected to prevent branching or other undesirable side reactions from occurring during synthesis. Oligonucleotides are synthesized on solid supports. Typically, the support is a small column filled with control pore glass (CPG), polystyrene or a membrane. The oligonucleotide is usually synthesized from the 3’ to the 5’. The synthesis begins with the addition of a reaction column loaded with the initial support-bound protected nucleotide into the column holder of the synthesizer. The first nucleotide building block or monomer is usually anchored to a long chain alkylamine-controlled pore glass (LCAA-CPG).

    A schematic diagram general outline the solid phase oligonucleotide synthesis of a dinucleotide is illustrated below.

    Phosphoramidite method
    Figure 11: Diagram of the workflow for the phosphoramidite method.

    The phosphoramidite approach to oligonucleotide synthesis proceeds in four (4) steps, which are schematically outlined in figure 11. Automated synthesis is done on solid support, usually controlled pore glass (CPG) or polystyrene. Synthesis is initiated with cleavage of the 5’-trityl group by brief treatment with dichloroacetic acid (DCA) dissolved in dichloromethane (DCM). Next, the monomer activated with tetrazole is coupled to the available 5’-hydroxyl resulting in a phosphite linkage. Subsequent phosphite oxidation by treatment with iodine using a THF/pyridine /H2O solution yields a phosphate backbone. The capping step with acetic anhydride, which terminates undesired failure sequences, completes the cycle of oligonucleotide synthesis.

    As figure 12 illustrates a typical synthesis cycle includes a condensation, a capping, an oxidation, and a cleavage or deprotection step. In general, automated DNA oligonucleotide synthesis produces a single-stranded oligonucleotide product per column.

     Phosphoamidite Oligonucleotide Solid
    Figure 12: A summary of the Phosphoamidite Oligonucleotide Solid Phase Synthesis Method. Removal from the support and final base deprotection process

    After the final sequence has been assembled, the oligomer must be removed by cleaving it from the support and fully deprotected prior to its use. A 90 minute treatment with ammonium hydroxide at room temperature can be used to cleave the oligomer from the support and to deprotect the phosphorous by β–elimination of the cyanoethyl group. The acetyl capping groups and the base protecting groups are more difficult to remove and a 24 hour treatment at room temperature or an overnight treatment at 55 °C with ammonium hydroxide allows for effective removal of these groups.

    After cleavage and deprotection, the resulting crude mixture contains the product oligomer, possible truncated failure sequences with free 5’hydroxyl ends, byproducts of deprotection, and silicates from hydrolysis of the glass support. Different purification methods can be used to separate the product oligonucleotide from the contaminating species.

    b) H-Phosphonate method.

    The first internucleotide reactions employing H-phosphonates were reported in the 1950s (Hall et al., 1957), and the first solid supported oligonucleotide synthesis was described in the early 1970s (Kabachnik et al., 1971), where the reported coupling efficiency was between 46 and 80%. The modern H-phosphonate method involves the use of triethylamine (TEA) or 1,8-Diazabicyclo[5.4.0]undec-7-ene salts, or DBU salts of corresponding 3’-H-phosphonate monomers, which need to be activated with the appropriate acetyl chloride followed by coupling of activated O-acetyl phosphonate to the 5’-hydroxyl of the previous nucleoside attached to solid support (Figure 13). Isopropyl-H-phosphonate together with the same activator is used for the capping step of the cycle.

    H-Phosphonate method
    Figure 13: Diagram depicting the H-Phophonate method.

    The resulting poly-H-phosphonate backbone can be oxidized after the final oligonucleotide is assembled from solid support in one step. The coupling efficiency of H-phosphonate synthesis is usually 94-95%, which is quite low compared to the phosphoramidite method. This approach is mainly used in cases when the desired modification of the phosphorous backbone can be done only through an H-phosphonate intermediate such as by using boranophosphates (Sergueev & Shaw, 1998) or phosphoramidates (Froehler, 1986), or others.

    c) Phosphotriester method.

    The phosphotriester approach (Letsinger & Mahadevan, 1965, 1966) is another successful method of oligonucleotide synthesis on solid support. Usually the coupling phosphorylating agent is an activated HOBt phosphate ester (Figure 14).

    Phosphotriester method
    Figure 14: Diagram of the phosphotriester approach.

    II. RNA synthesis.

    Automated RNA synthesis on solid support was introduced a decade later than DNA synthesis and many disappointing results had to be overcome leading to a difficult history of development. The use of one extra 2’-hydroxy group makes this chemistry magnitudes more difficult compared to conventional DNA synthesis. The protective group of 2’ hydroxyl in ribose must remain intact both during the synthesis and base deprotection step. The last property of the protection is critical due to the hydrolytic instability of RNA in basic conditions. That is the reason why all modern methods of RNA synthesis that employ different RNA phosphoramidite monomers have protective groups at the 2’ hydroxyl group. This protective group needs to be stable in basic conditions during base deprotection and can be easily removed to completion on the next processing step using different or orthogonal conditions.

    TBDMS Phosphoramidites

    TBDMS Phosphoramidites are the first type of RNA monomers that have been developed by Kelvin Ogilvie group at McGill University in mid 1980s. That type of monomers has a 2’- tert-butyldimethylsilyl (TBDMS) protecting group and it is the most common type of building blocks used in solid phase RNA synthesis. The protecting group is stable in methylamine solution and less stable in ammonia.

    Disadvantage : The phosphoramidite building blocks require longer coupling times compared to other RNA building blocks.

    TBDMS Phosphoramidites
    Figure 15: TBDMS phophoramidites
    TOM phosphoramidites

    Xeragon AG introduced TOM phosphoramidites about six years ago. This type of monomer has a 2’-triisopropyl-silyl-oximethyl (TOM) protecting group, which is quickly removed by treatment with tetrabutylammonium fluoride (TBAF). The biggest benefit of this chemistry is that it allows the synthesis of longer up to 100 mer oligonucleotides.

    Disadvantage :It is commercially available only for research applications.

    TOM phophoramidites
    Figure 16: TOM phophoramidites
    Fpmp Phosphoramidites

    Fpmp Phosphoramidites have 2’-fluorophenyl-methoxy-pipiridyl (Fpmp) protection groups. These phophoramidites were developed by C. B. Reese in the late 1980s and the first commercially available RNA phosphoramidites were introduced by Cruachem Ltd. in the early 1990s. One of the main advantages of this chemistry is that the protecting group is useful for RP HPLC and stays intact until all post-synthetic manipulations are done. Most importantly this protection group prevents RNA from cleavage by RNases.

    Disadvantage :It is commercially available only for research applications.

    Fpmp phophoramidites
    Figure 17: Fpmp phophoramidites
    ACE Monomers

    ACE monomers were developed in M. Caruthers’s group and introduced in the mid 1990s by Dharmacon, Inc. This type of RNA monomers have a 2’-acetylethoxy orthoester (ACE) protecting group and are 5’-hydroxyl protected with a silyl protecting group. This approach differs from conventional DMT protection. The ACE orthoester group is removable using mild acidic conditions. In order to avoid complications as observed in the case of Fpmp chemistry, ACE monomers have been designed with special 5’ silyl protecting groups that are removable in the presence of tetra-n-butylammonium fluoride (CH3CH2CH2CH2)4N+F-) or TBAF.

    Disadvantage :Contrary to DMT, 5’-silyl protection is not a convenient colored marker and cannot be used for quantitative and qualitative synthesis evaluation.

    ACE Monomers
    Figure 18: ACE monomers

    III. Synthesis of modified oligonucleotides

    The synthesis of modified oligonucleotides involves the selection of the best strategy to design and plan the needed synthetic approach or pathway. The following paragraph shows how to develop a synthetic strategy and introduce desired modification into oligonucleotide using manual phosphoramidite coupling and how to optimize post synthetic labeling with an activated ester.

    a) Strategy of synthesis

    A good way to plan a synthesis is to start with a retro-synthetic analysis. This approach starts with determining what the final product should be and going backwards along the synthetic route to determine what kind of reagents have the appropriate protecting groups needed for the synthesis. An example is shown below. The synthetic path needs to be designed using modifications that do not interfere with other functional groups. That is, the best orthogonal conditions will need to be established.

    b) On-Support modifications

    The easiest on support modifications known is the oxidation of H-phosphonates or phosphite triesters using elemental sulfur or disulfides to afford phosporothioates. There are a number of modifications of the phosphate backbone, which can be done while the oligonucleotide is attached to solid support. Most of them require special phosphoramidites, such as the use of alkyl phosphonates and phosphate triesters, or others. Some of them require an H-phosphonate backbone for the transformation. Examples are the use of boranophosphates (Sergueev & Shaw, 1998) or phosphoramidates (Froehler, 1986).

    Internal modifications on oligonucleotides can be done using either corresponding extendible amidites or using an asymmetrical branch with a non-extendible amidites and any 5’ modifications can be introduced using any type of monomers using an automated synthesizer. In addition any 3’ modification can be introduced using the appropriate solid support. The reversed synthesis from the 5’ to 3’ end can be employed for 3’ modifications if a solid support is not available but the needed phosphoramidite is. The same approach is applicable for 5’ modifications when the modifications are only available on a solid support. Furthermore, phosphoramidite chemistry is not the only chemistry that can be performed using solid support. Some peptide chemistry methods are also compatible with oligonucleotide synthesis methods. For example NHS esters or similar activated esters can be attached to unblocked aliphatic amino-linkers on solid support. In this case the desired molecular probe or reporter molecule has to be stable in basic conditions used during cleavage and base deprotection.

    c) Post-synthetic modifications.

    The most common chemo-selective reagents for post synthetic oligonucleotide modifications, for example to introduce different molecular probes and markers, are N-hydroxysuccininidyl activated esters. This type of activated esters reacts very selectively with aliphatic primary amines and is very stable in aqueous buffers at pH 8-9 towards hydrolysis. However, the following three very important issues in post-synthetic NHS labeling need to be addressed. First, the pH of the reaction mixture needs to be maintained at pH 8-9 during the coupling reaction. Second, the correct salt form of the oligonucleotide needs to be used, since ammonia or amino contaminants can also react with NHS esters to form unwanted different by-products. Therefore it is very important to exchange all ammonia ions with other types of counter ions such as lithium or sodium ions. This can be achieved either by precipitation of the oligonucleotides with lithium or sodium ions or by using HPLC with the corresponding buffers. Third, another important requirement for a successful reaction is to make sure that the NHS ester is completely soluble in the reaction mixture of the corresponding buffer and DMSO or other organic solvent suitable for the reaction used. This is needed because the precipitated ester is unreactive.

    This method can be used not only for conjugation of DNA to small molecules, but also for DNA-protein conjugations (Jablonski et al. 1986).

    Michael addition is another useful selective reaction for oligonucleotide post synthetic modifications. Maleimide derivatives and sulfhydryl modified oligonucleotides are usually employed for this method. This approach has been used for conjugation DNA and proteins as well (Ransom Hill Bioscience, Inc, Technical Bulletin).

    There are a few other chemoselective methods, which have been used for DNA–protein conjugations and the attachment of modified oligonucleotides to solid supports, such as those involving aldehydes and hydrazides, disulfides and thioesters, etc. (see IDT Technical Bulletins). All these methods are very useful for the introduction of post-synthetic oligonucleotide modifications.

    d) Automated DNA/RNA synthesizer

    One of the earliest, or the earliest, DNA/RNA synthesizer can be viewed at the Smithsonian Institution in Washington DC. The DNA/RNA synthesizer model 394 is made out of steel, glass, plastic and insulated wire. It has the measurements: 50 cm x 66 cm x 46 cm; 19 11/16 in x 26 in x 18 1/8 in.

    This Model from Applied Biosystems, Inc. was on the market from 1991 to 2007. DNA/RNA synthesizers can produce short single strands of nucleotides known as oligonucleotides which can be further linked together to create longer strands of DNA or RNA. Because of the automated approach it is now often easier to make stretches of DNA or RNA with a synthesizer than it is to isolate the same stretches of DNA or RNA from natural sources. In addition, synthetic oligonucleotides can also be created with slight changes from the naturally occurring forms, such as the insertion of artificial nucleotides, allowing researchers to study the impact of modified molecules.

    Figure 21: DNA/RNA synthesizer model 394.

    Figure 22 shows pictures of typical reaction columns containing either activated or monomer loaded CPG supports as well as pictures of automated oligonucleotide synthesizers. Using this type of supports succinylated oligonucleotides or their monomers can be coupled to aminophenyl- or aminopropyl-derivatized glass surfaces, and disulfide-modified oligonuclotides or their monomers can be immobilized onto a mercaptosilanized glass support using a thiol/disulfide exchange reaction or through chemical cross-linkers.

    Figure 22: Icons of reaction columns and automated oligonucleotide synthesizers are shown. (Left) Different types of columns, color coded. (Middle) Columns mounted into the holder of the synthesizer. (Right) ABI 3900 and Expedite High-Throughput DNA/Oligo Synthesizers.

    Table 1: Some milestone in DNA/RNA solid phase oligonucleotide synthesis.

    Year / decade Event Researcher(s)
    1955 First published account of the chemical synthesis of an oligonucleotide. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage. Phosphotriester method. Michelson and Todd
    1958 Improved and general method for the synthesis of ribo-and deoxyribo-nucleoside-5' triphosphates. Khorana and co~workers first used the tetrahydropyranyl group (Thp) as 2' -protection in the synthesis of mononucleotides. Smith and Khorana
    1962
    1963
    On-off protection for sequential oligonucleotide synthsis.Stable phophorylated nucleosides are coupled to desired nucleoside when activated. Korana lab
    Smith et al.
    Schaller et al.
    1960s Robert Letsinger devised a method for assembling oligos via solid phase chemistry. Oligonucleotides are constructed by linking chemical building blocks onto polymer beads. This, together with slight adjustments to Khorana’s original protocol, simplified the reaction so that the first automated machines to perform oligonucleotide synthesis could be built in the late 1970s. Letsinger
    1963 Stepwise synthesis of ribooligonucleotides. Lapidot & Khorana
    1965, 1966 Phosphotriester approach Lapidot & Khorana
    1980s Further adjustments to the reaction made it possible for someone without a great deal of experience in chemical preparation to operate the machines, opening up their use to a wider audience and increasing their commercial viability.  
    1985 RNA synthesis using 2'-O-(tert-butyldimethylsilyl) protection Ogilvie
    1986 H-Phosphonate Diester Intermediates Froehler
    1989 Applied Biosystems started marketing oligonucleotide synthesizers. ABI
    1998 H-Phosphonate Approach Sergueev
    1991 to 2007 DNA/RNA synthesizer model 394. This model is considered as the second wave of Applied Biosystems’s DNA/RNA synthesizers which consumed chemicals more efficiently than the previous model and could synthesize up to four oligos at one time. ABI
    Reference

    Abbreviations and Symbols for the Description of Conformations of Polynucleotide Chains. IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN): Recommendations 1982. www.chem.qmul.ac.uk/iupac/misc/pnuc1.html

    Tatyana Abramova; Review: Frontiers and Approaches to Chemical Synthesis of Oligodeoxyribonucleotides. Molecules 2013, 18, 1063-1075; doi:10.3390/molecules18011063.

    Avery, OT, MacLeod, CM and McCarty, M (1944). Studies on the chemical nature of the substance inducing transformation of Pneumococcal types. J. Exp. Med. 79, 137-158.

    Willi Bannwarth; Synthesis of Oligodeoxynucleotides by the Phosphite-Triester Method Using Dimer Units and Different Phosphorous-Protecting Groups. Helvetica Chimica Acta, Volume 68, Issue 7, pages 1907–1913, 13 November 1985.

    J.C. Biro, B. Benyó, C. Sansom, Á. Szlávecz, G. Fördös, T. Micsik, and Z. Benyó; A common periodic table of codons and amino acids. Biochemical and Biophysical Research Communications 306 (2003) 408–415.

    Marvin H. Caruthers; The Chemical Synthesis of DNA/RNA: Our Gift to Science. January 11, 2013 The Journal of Biological Chemistry, 288, 1420-1427.

    F Eckstein. Oligonucleotides and analogues: A practical approach. Edited by F Eckstein. Series editors: D. Rickwood and B.D. Hames. IRL PRESS. Oxford University Press. 1991.

    Froehler, B.C. Deoxynucleoside H-Phosphonate Diester Intermediates in the Synthesis of Internucleotide Phosphate Analogues Tetrahedron Lett. 1986, 27, 5575-5578.

    P. T. Gilham and H. G. Khorana; Studies on Polynucleotides. I. A New and General Method for the Chemical Synthesis of the C5″-C3″ Internucleotidic Linkage. Syntheses of Deoxyribo-dinucleotides. J. Am. Chem. Soc., 1958, 80, 6212–6222.

    Hall, R.H., Todd, A., and Webb, R.F. 1957. Nucleotides. Part XLI. Mixed anhydrides as intermediates in the synthesis of dinucleoside phosphates. J. Chem. Soc. 3291-3296.

    Jablonski, E.; Moomaw, E.W.; Tullis, R.H. & Ruth, J.L. Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucl. Acid Res. 1986, 14, 6115-6128.

    Kabachnik, M.M.; Potapov, V.K.; Shabarova, Z.A. & Prokofiev, M.A. Dokl. Acad. Nauk SSSR 1971, 201, 858-861.

    H. G. Khorana, G. M. Tener, J. G.Moffatt and E. H. Pol, Chem. Ind., 1956, 1523.

    H. G. Khorana, W. E. Razzell, P. T. Gilham, G. M. Tener and E. H. Pol; SYNTHESES OF DIDEOXYRIBONUCLEOTIDES. J. Am. Chem. Soc., 1957, 79, 1002–1003.

    Y. Lapidot, H. G. Khorana; Studies on Polynucleotides. XXVIII. The Specific Synthesis of C3″-C5″-Linked Ribooligonucleotides (4). The Stepwise Synthesis of Uridylyl-(3″ → 5″)-adenylyl-(3″ → 5″)-uridylyl-(3″ → 5″)-uridine. J. Am. Chem. Soc., 1963, 85 (23), pp 3852–3857.

    Y. Lapidot, H. G. Khorana; Studies on Polynucleotides. XXIX. The Specific Synthesis of C3″-C5″-Linked Ribooligonucleotides(5). Homologous Adenine Oligonucleotides. J. Am. Chem. Soc., 1963, 85 (23), pp 3857–3862.

    Letsinger, R.L. & Mahadevan, V. J.; Oligonucleotide Synthesis on a Polymer Support. Am. Chem. Soc. 1965, 87, 3526-3527.

    Letsinger, R.L. & Mahadevan, V. J. ; Stepwise Synthesis of Oligodeoxyribonucleotides on an Insoluble Polymer Support. Am. Chem. Soc. 1966, 88, 5319-5324.

    McBride, L.J. & Caruthers, M.H. An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides. Tetrahedron Lett. 1983, 24, 245-248.

    A. M. Michelson and Alexander R. Todd; Nucleotides part XXXII. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage. J. Chem. Soc., 1955, 2632-2638. DOI: 10.1039/JR9550002632.

    J. G. Moffatt, H. G. Khorana; D-XYLOSE-3-PHOSPHATE. J. Am. Chem. Soc., 1956, 78 (4), pp 883–884.

    Operation Manual. MilliGen/Biosearch Cyclone™ Plus DNA Synthesizer.

    Usman, N.; Ogilvie, K. K.; Jiang, M. Y.; Cedergren, R. J.; The automated chemical synthesis of long oligoribuncleotides using 2'-O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNA. J. Amer. Chem. Soc. 1987, 109 (25): 7845–7854

    D. H. Rammler , H. G. Khorana; Studies on Polynucleotides. XX.1 Amino Acid Acceptor Ribonucleic Acids (1). The Synthesis and Properties of 2″ (or 3″)-O-(DL-Phenylalanyl)-adenosine, 2″ (or 3″)-O-(DL-Phenylalanyl)-uridine and Related Compounds. J. Am. Chem. Soc., 1963, 85 (13), pp 1997–2002.

    Reese, C.B. & Tompson, E.A.; A new synthesis of 1-arylpiperidin-4-ols. J. Chem. Soc. Perkin Trans. I, 1988, 2881-2885.

    Roy, S. and Caruthers, M.; Synthesis of DNA/RNA and their analogs via phosphoramidite and H-phosphonate chemistries. Molecules 2013, 18, 14268-14284.

    The RNA World. Second Edition. Ed. R. F. Gesteland, T. R. Cech, J. F. Atkins. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York. Monograph 37, 1999.

    Subhadeep Roy and Marvin Caruthers; Synthesis of DNA/RNA and Their Analogs via Phosphoramidite and H-Phosphonate Chemistries. Molecules 2013, 18, 14268-14284; doi:10.3390/molecules181114268.

    Scaringe, S.A.; Wincott, F.E. & Caruthers, M.H.; Preparation of 5′‐Silyl‐2′‐Orthoester Ribonucleosides for Use in Oligoribonucleotide Synthesis. J. Am. Chem. Soc. 1998, 120, 11820-11821.

    H. Schaller, G. Weimann , B. Lerch , H. G. Khorana; Studies on Polynucleotides. XXIV. The Stepwise Synthesis of Specific Deoxyribopolynucleotides (4). Protected Derivatives of Deoxyribonucleosides and New Syntheses of Deoxyribonucleoside-3″ Phosphates. J. Am. Chem. Soc., 1963, 85 (23), pp 3821–3827.

    H. Schaller, H. G. Khorana; Studies on Polynucleotides. XXV. The Stepwise Synthesis of Specific Deoxyribopolynucleotides (5). Further Studies on the Synthesis of Internucleotide Bond by the Carbodiimide

    Method. The Synthesis of Suitably Protected Dinucleotides as Intermediates in the Synthesis of Higher Oligonucleotides. J. Am. Chem. Soc., 1963, 85 (23), pp 3828–3835.

    H. Schaller, H. G. Khorana ; Studies on Polynucleotides. XXVII. The Stepwise Synthesis of Specific Deoxyribopolynucleotides (7). The Synthesis of Polynucleotides Containing Deoxycytidine and Deoxyguanosine in Specific Sequences and of Homologous Deoxycytidine Polynucleotides Terminating in Thymidine. J. Am. Chem. Soc., 1963, 85 (23), pp 3841–3851.

    Sergueev, D.S. & Shaw, B.R.; H-Phosphonate Approach for Solid-Phase Synthesis of Oligodeoxyribo-nucleoside Boranophosphates and Their Characterization. J. Am. Chem. Soc. 1998, 120, 9417-9427.

    Smith, M., Rammler, D.H., Goldberg, I.H. and Khorana, H.G. (1962). Studies on Polynucleotides. XIV. Specific Synthesis of the C3'-C5' Interribonucleotide Linkage. Synthesis of Uridylyl-(3'->5')-Uridine and Uridylyl-(3'->5')-Adenosine. J. Amer. Chem. Soc. 84, 430-440.

    Smith, M. and Khorana, H.G. (1958). Nucleoside polyphosphates. VI. An improved and general method for the synthesis of ribo-and deoxyribo-nucleoside-5' triphosphates. J. Amer. Chem. Soc. 80, 1141-1145.

    Sproat BS.; RNA synthesis using 2'-O-(tert-butyldimethylsilyl) protection. Methods Mol Biol. 2005;288:17-32.

    G. M. Tener, H. G. Khorana, R. Markham, E. H. Pol; Studies on Polynucleotides. II.1 The Synthesis and Characterization of Linear and Cyclic Thymidine Oligonucleotides. J. Am. Chem. Soc., 1958, 80 (23), pp 6212–6222.

    G. Weimann, H. Schaller , H. G. Khorana; Studies on Polynucleotides. XXVI. The Stepwise Synthesis of Specific Deoxyribopolynucleotides (6). The Synthesis of Thymidylyl-(3″ → 5″)-deoxyadenylyl-(3″ → 5″)-thymidylyl-(3″ → 5″)-thymidylyl-(3″ → 5″)-thymidine and of Polynucleotides Containing Thymidine and Deoxyadenosine in Alternating Sequence. J. Am. Chem. Soc., 1963, 85 (23), pp 3835–3841.

    Winnacker, Ernst L.; From genes to clones: introduction to gene technology. VCH, 1987.

    R. S. Wright, H. G. Khorana; Phosphorylated Sugars. I. A Synthesis of β-D-Ribofuranose 1-Phosphate. J. Am. Chem. Soc., 1956, 78 (4), pp 811–816.


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     Aldehyde modified oligonucleotides, their broad utility in bio-conjugation applications and surface attachment methods.

    In recent times both basic and applied molecular biology studies have made extensive use of modified oligonucleotides as tools in the study of gene regulation, drug discovery and diagnostics.  In particular, aldehyde modified oligonucleotides have been used in applications such as bio-conjugation1 and micro-array2,3 in hybridization-based assays. 

    The conjugation reaction between aldehyde and hydrazides, hydrazines, semicarbazides or aminooxy fynctionalized synthons is highly chemically selective and efficient.1-3  It was shown that the resulting Schiff-base is relatively stable linker,2 however it can be stabilized further by reduction to the corresponding hydrazine.4  Most methods are orthogonal to the most popular conjugation methods utilizing Michael addition reaction or activated carboxylates.  Therefore the aldehyde functional group will not interfere with those reactions and can be used, when other functional groups involved in the parallel process.

    Bio-synthesis offers not only wide varieties of 3’-, 5’- and internally aldehyde modified oligonucleotides and also their conjugates with peptides, proteins and antibodies.1

     

    References:

    1.      http://www.biosyn.com/Bioconjugation.aspx

    2.      M. A. Podyminogin, E. A. Lukhtanov and M. W. Reed Nucleic Acid Res., 2001, 29, 5090-5098.

    3.       S. Raddatz, J. Mueller-Ibeler, J. Kluge, L. Wäß, G. Burdinski, J. R. Havens, T. J. Onofrey, D. Wang, and M Schweitzer Nucleic Acid Res., 2002, 30, 4793-4802.

    4.      E. N. Timofeev, A. D. Mirzabekov, S. V. Kochetkova and V. L. Florentiev Nucleic Acid Res., 1996, 24, 3142-3148.


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    Epigenetics and the inheritance of epigenetic defects

    By Klaus D. Linse

    Have you ever wondered if where you grew up and where you lived or live now, what you eat and what you do had or has an influence on your health? Evidence is mounting that this is the case. Our genes seem to have a memory of our past. Research indicates that the nutritional fate of our grant parents may have left their mark on our genes. How can this be? Bee keepers know how to tell a worker bee from a queen bee. But what makes a worker bee look different from a queen bee? It’s her sister. Unlike in humans, royalty is not inherited in honey bees. The honeybee queen bee and worker bees are genetically identical but the queen larvae get feed a special diet – royal jelly – in large quantities and for long periods to make the difference. The worker bees become slaves to the queen. Something is happening on top of the bee’s genome. Alas, the epigenome came to pass. The honeybee’s epigenome has been studied by Lyko et al. recently and the methylome, the methylated genome, of the brains of worker bees and queen bees has been published in 2010. The pictures below show the two different bees and a depiction of the methylome.

    bee bee methylome
    A worker bee The queen bee The methylome

    Figure 1: A picture of a worker bee (left), the queen bee (middle) and an icon of the methylome is depicted here.

    In a paper published in the journal Science in the year 1987 Robin Holliday reports that there is plenty of evidence now originating from many different sources that indicating that control of gene expression in higher organisms is related to the methylation of cytosine in DNA. Furthermore, the pattern of methylation appears to be is inherited. Scientists observed that the loss of methylation, for example resulting from DNA damage, will lead to heritable abnormalities in gene expression. Robin Holliday noted that these events may be important in oncogenesis and aging. Robin further proposed that epigenetic defects in germ line cells due to loss of methylation can be repaired by recombination during meiosis but that some maybe transmitted to offsprings.

    The term “epigenetics” was coined by Waddington (1942) to refer to the study of the “causal mechanisms” by which “the genes of the genotype bring about phenotypic effects.” Furthermore he stated that “Epigenetics has different meanings for different scientists”. In the mind of a molecular biologist that may involve the study of heritable changes of the DNA that can be observed during mitosis that cannot be explained by changes in DNA sequence. These changes include DNA methylation, histone modification and others. For other scientists epigenetics could refer to interactions of cells and cell products that lead to morphogenesis and differentiation. Simply speaking, epigenetic is the study of genetic effects on the phenotype that are not caused by alteration of the DNA sequence including heritable effects on a genes or chromosomes function that is not accompanied by a change in the DNA sequence. As we will see the notion of epigentics has a long history. However, only in the last 25 years did it become an intensely studied scientific problem waiting to be solved. The figure below shows the explosive increase in publications for epigenetics in general and the epigenetics related to cardiovascular disease from 1995 to 2010. Due to the immense list of publications in the field only some major representative papers will be reviewed here.

    epigenetic mechanisms

    Figure 2: Illustration of some epigenetic mechanisms

    Epigenetic publications

    Epigenetic publications: total number and number related to cardiovascular disease. PubMed Search performed on October 30th 2010 using the search terms (epigenetics OR DNA methylation OR histone modifications) for “epigenetics”; and (cardiovascular OR cardiovascular disease) for “cardiovascular disease”

    *2010 data were projected by multiplying the 2009 publication numbers by the 2007–2009 yearly relative increment. As of October 30th 2010, PubMed listed 6,586 publications on epigenetics and 280 on epigenetics & cardiovascular disease. [Source Baccarelli et al., 2010]

    Figure 3: Increase in research publications covering epigentics since 1995.

    According to Michael J. Meany phenotype emerges only from the interaction of gene and environment. The function of a gene cannot be separated from its cellular environment. Every trait results from the interaction of a gene with the environment. The development of defensive responses to a threat as a maternal effect is a well established theme in biology. Evidence for transgenerational, maternal effects has been published for two animal models – one for a plant and the other for an insect. If a plant gets provoked, say by getting eaten by an insect, it usually reacts with an increase in the synthesis of defensive molecules such as mustard oil glycosides in the case of the radish. These defenses defend against the next attacks. The seedlings derived from caterpillar-damaged radishes showed a significant change in glycosinolate profiles and altered trichrome expression. Since only the mother plants had been exposed to the caterpillars but not the seedlings, these changes have to be adaptive. There are more examples available such as the capacity for night flight in grasshoopers, the tail length of lizards, and the helmet size of water flies and many more are getting discovered the more scientists look for them. These are all determined by maternal effects acting through yet unknown mechanisms. In these examples, traits of the parents are transmitted to the offsprings in a nongenomic manner. The environmental experience of the mother is inherited through an epigenetic mechanism of inheritance causing a phenotypic variation in the offspring. Studies off rats looking for maternal effects revealed that naturally occurring variations in maternal care are associated with individual differences in hypothalamic-pituitary-adrenal (HPA) axis responses to stress. For example the adult offspring of a high licking and grooming mother showed increased hippocampal glucocorticoid receptor expression and enhanced glucocorticoid feedback sensitivity compared to the offspring of a low licking and grooming mother. The first offspring also showed a decrease in hypothalamic corticotrophin-releasing factor (CRF) expression and a more modest HPA response to stress. The result is that the adult offspring rats from frequently licking and grooming mothers are in general behaviorally less fearful and show more modest HPA responses to stress than the offsprings of low licking and grooming mothers. How is this accomplished? In vivo and in vitro studies suggest that the glucocorticoid receptor gene expression is altered through increased hippocampal serotonin (5-HT) activity at 5-HT7 receptors. The increased 5-HT activity results in an increase in the expression of the transcription factor, nerve growth-inducible factor A (NGFI-A) in the hippocampus. The non-coding exon 1 region of the hippocampal glucocorticoid receptor includes a promoter region, exon 17, containing a binding site for NGFI-A. Noncoding regions of the DNA do not code for functional gene products – proteins - and usually contain sequences that regulate the expression of the “downstream” coding segment. For example, exon 1 contains several promoter sequences that can alter gene expression. The exon 17 sequence functions as a promoter in neurons and is more active in the offspring of high licking and grooming mothers, suggesting that the use of this promoter is enhanced as a function of maternal care. Transcription factors such as nerve growth-inducible factor A regulate gene expression and provide a cellular interface between environment and gene. Many details of our behavior and appearance appear to be determined by gene regulation. A striking example of the power of gene regulation is seen in agouti mice (see figure 4), in which genetically identical twins can look entirely different in both color and size. For example, one mouse may be small and brown, but her twin sister may be obese and yellow. Another genetically identical sister may have a mottled look with both fur colors present but may fall in the middle of the weight range. The genome of each of these mice is the same, but the gene expression obviously differs. How can that be? Waterland and Jirtle in 2003 studied the influence of nutrition on adult metabolism in yellow agouti (Avy) mice and showed that research on epigenetic changes resulting from the environment can give clues into obesity in mice--and humans. Their results showed that dietary methyl supplementation of a/a female mice with extra folic acid, B12, choline, and betaine alter the phenotype of their Avy/a offspring via increased CpG methylation at the Avy locus. This locus is a transposable gene element. We conclude, the epigenome is what makes the difference.

    Historical milestones leading to the notion of epigenetics
    c. 300bc Hippocrates thought that humans inherited little parts from all parts of their parents. Whereas Aristotle (around 384-322 bc) thought that humans grew from unformed blobs that developed inside the mother because of the father. Other thoughts of the time were that humans were fully-formed to start with.
    1651

    William Harvey (1578-1657) further explored what Aristotle had put forward. He dissected deer and chicks to understand how embryos form. He came to the conclusion that embryos developed gradually from an egg, rather than from tiny fully-formed bodies.

    1665 Cells were described and named by Robert Hooke
    1859 Concept and facts of evolution were postulated by Charles Darwin
    1865 In Austria a monk was planting peas and watched how parents passed on their features to the next generation of peas. He established the rules of inheritance, which are the basis of genetics today. His name was Gregor Mendel (1822-1884). Alas the “Mendalian rules” were found in a garden in a monastery in Bruenn (now Brno in the Czech Republic).
    1869 “Nuclein,” a new acidic, phosphorus-containing, long molecule was found by F. Miescher
    1876 The notion of “Nature and nurture” was described by F. Galton.
    1892 August Weissmann (1834-1914) and others recognized that genetic information was stored in the nucleus of a cell. It was thought that cells start with the same information then become more specialized by losing material when they divide.
    1902 Hans Spemann (1869-1941) agreed with Weissmann but argued that cells don’t lose information; they merely switch it off. He used a strand of his baby’s hair to split a salamander egg in two. The result was two salamanders. Spemann was the pioneer of the modern cloning technology.
    1906 The term “genetics” was proposed by W. Bateson.
    1915 It was established that Genes are located on chromosomes leading to the “chromosomal theory of inheritance”. The first genetic linkage in invertebrates was found as well and the term “intersex” was coined.
    1926 Enzymes are proteins
    1942 Conrad Waddington (1905-1975) coined the term epigenetics. He thought about development and inheritance in terms of the cross-talk between genetic information and the environment
    1953 James Watson and Francis Crick (1916- 2004) describe the structure of the DNA double helix in terms of the four letters of the genetic alphabet. DNA is now recognized as the hereditary genetic material.
    1980 on The gene sequencing revolution begins. Science takes on the view that humans are the sum of their gene sequences.
    1990 on Epigenetics research takes off. Scientists realize that it is not just DNA sequences which control a human’s biological make-up. DNA methylation and histone modifications are recognized as important regulators of gene activity.
    1998 RNA interference, RNAi was discovered. Human embryonic stem cells were found.
    2000 to 2001 The first draft sequence of the human genome was published: Greg Venter et al. published a paper in Science in 2001 that contained the sequence of the euchromatic portion of the human genome. A 2.91-billion base pair (bp) consensus sequence was generated by the whole-genome shotgun sequencing method. The sequence assemblies covered the euchromatic regions of the human chromosomes. The data showed that more than 90% of the genome is in scaffold assemblies of 100,000 bp or more, and 25% of the genome is in scaffolds of 10 million bp or larger. Analysis of the genome sequence revealed 26,588 protein-encoding transcripts and an additional ∼12,000 computationally derived genes. Almost half the genes are dispersed in low G+C sequence separated by large tracts of apparently noncoding sequences and only 1.1% of the genome is spanned by exons, whereas 24% is in introns, with 75% of the genome being intergenic DNA. Duplications of segmental blocks are abundant throughout the genome. Less than 1% of all SNPs resulted in variation in proteins

    What happens when cells develop into different cell types using the same genome as a template?

    Let us first review what we know so far: Human life begins with a single cell. This embryonic “stem cell” contains all the genetic information needed to develop into a full-grown adult, the genome. Through repeated cell divisions the cell eventually multiplies into ten of trillions of cells. Each cell contains a complete copy of the genome. However, despite having the same genetic information these cells develop into hundreds of different cell types that make up the human body. To find out how this works the field of epigenetics came to pass.

    A process called mitosis splits a single cell into two cells with identical genetic information. Each cell is capable to develop into different cell types, e.g. into blood cells, neurons or others. We now think that the epigenome determines what type of cell a stem cell will become. Each cell has the same copy of the instruction manual but a brain cell, for example, may only use certain chapters, say to build synapses. The epigenome of each cell tells the cell what chapter to read. Further, we also know now that DNA coils around proteins called histones forming the nucleosome. The helical DNA double strands are packed tightly around the histones inside the nucleus of the cell. The nucleosome is coiled further into a rope like structure called chromatid, which itself is packaged into the chromosome. The epigenome controls access to the genes by attaching molecular caps called methyl groups at certain points of the genes to block them. Histones can coil so tightly around the DNA so that some genes become unreadable. Methyl groups attached to base pairs of a gene changes the expression of the gene. The result is that the DNA of the cells is identical but the epigenetic counterpart is not. Cells perform different functions because now they have different patterns of methyl groups and histones controlling which genes are expressed. DNA methylation is a stable, epigenomic mark occurring at cytosine nucleotides often found within promoter sequences. Histone deacetylases (HDAC) are enzymes that remove acetyl groups (O=C-CH3) from ε-N-acetyl lysines on a histone. The removal of the acetyl group now causes the histones to wrap the DNA around themselves more tightly. DNA methylation is therefore associated with a stable suppression in gene transcription and represents a mechanism to turn genes off.

    So what exactly is epigenetics?

    Epigenetics is defined as the study of

    • Changes in gene expression occurring in organisms with differentiated cells, and the mitotic inheritance of given patterns of gene expression.
    • The nuclear inheritance that is not based on changes in DNA sequence.
    • "the study of the mechanisms of temporal and spatial control of gene activity during the development of complex organisms." as defined by Robin Holliday.
    • The Greek prefix epi- in epigenetics implies features that are "on top of" or "in addition to" genetics; thus epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance.
    • The "epigenome" is used in parallel to the "genome" referring to the overall epigenetic state of a cell. The "epigenetic code" has been used to describe the set of epigenetic features that create different phenotypes in different cells. An epigenomic map would be a representation of gene expression, DNA methylation and histone or other protein modification status of a particular genomic region or the whole genome.

    Adrian Bird reports in 2007 in “Perceptions in Epigenetics” that there are two classic epigenetic systems.

    A. There is the Polycomb and Trithorax (Polycomb/Trithorax) system, and

    B. DNA methylation.

    The Polycomb and Trithorax groups of proteins, named after mutants of the fruitfly Drosophila melanogaster, maintain repressed or active transcription states, respectively, of developmentally important genes. If they are absent the genes that specify the different segments of the fruitfly are initially expressed correctly but the gene pattern cannot be maintained. It appears that the Polycomb/Trithorax systems establish stable ’memorized’ gene-expression patterns that have been set up by other cellular mechanisms. It has been reported that Polycomb-imposed silencing can even be transmitted between fruitfly generations at low frequencies. Components of the two key Polycomb-system protein complexes have been identified and a close link with modification of the lysine residue at position 27 of histone H3 has been established. However, the mechanism by which silencing is transmitted between cell generations remains unknown. In vertebrates the methylated sequence is CG, which is paired with the same sequence on the opposite DNA strand. Methylation sites are transiently found on only one of the two DNA strands after DNA replication. CG methylation patterns can be copied between cell generations by the DNA methyltransferase DNMT1, which ‘completes’ hemimethylated but not unmethylated sites. DNA methylation is associated with stable gene silencing (for example, on the inactive X chromosome), either through interference with transcription-factor binding or through the recruitment of repressors that specifically bind sites containing methylated CG. Nuclear receptors can transduce environmental and metabolic signals into alterations in gene expression. The recruited coregulator molecules alter the structure of the chromatin. The following figure shows locations of CpG islands in a hypothetical gene. Methylated and unmethylated islands are depicted.

    Figure 5: Locations of CpG islands in a hypothetical gene. Methylated and unmethylated islands are shown here.

    In the year 2000 Allis and Strahl introduced the notion of the “Histone Code”. The researchers reported that a diverse array of post-translational modifications that often occur on tail domains of histone proteins has been well documented. Furthermore, in their paper they proposed that “distinct histone modifications, on one or more tails, act sequentially or in combination to form a 'histone code' that is read by other proteins to bring about distinct downstream events.” The conventional thinking at the time was that the modifications on the histones just strengthened or loosened the nucleosomes hold on DNA altering gene expression accordingly. The histone code hypotheses proposed instead that these epigenetic modifications represent a histone language that other proteins could read, write and erase and modify. If scientist could decipher this code they could predict events such as transcription, chromatin remodeling and silencing.

    In 2001 Jenuwein and Allis published a paper reporting on how to translate the histone code. Chromatin in all eukaryotes contains an array of posttranslational modifications. The majority of which are found on the amino-termini of histones. They proposed that histone proteins and their associated covalent modifications contribute to a mechanism that can alter the structure of chromatin. These modifications then lead to an inherited difference in transcriptional states. They can be viewed as “off” and “on” states that define the higher order structure of the centromers. Chromatin based events can lead to either gene activation or gene silencing. Differences in histone modifications are descriped as“euchromatic” (“on”) or “heterochromatic” (“off”). A schematic representation of the proposed model is depicted in figure 6.

    Schematic representation Schematic representation of a model of euchromatin and heterochromatin. Accessible or condensed nucleosome fibers contain different sets of acetylated (Ac), phosphorylated (P), and methylated (Me) histone amino-termini.

    Figure 6: Proposed model of euchromatin and heterochromatin (2001 Jenuwein and Allis) illustrating the “off” and “on” states that define the higher order structure of the centromers.

    In the nuclei of all eukaryotic cells, genomic DNA is found as a highly folded, constrained and compacted dynamic polymer. The DNA polymer strands are wound around histones and are compacted further with non-histone proteins. Chromosomal regions that remain transcriptionally inert are highly condensed in the interphase nucleus and can be observed in the cell as heterochromatic foci or as the “Barr body,” which represents the inactive X chromosome in female mammalian cells. Roughly two superhelical turns of DNA wrap around an octamer of core histone proteins in the nucleosome. The core histone proteins that form the octamer are H3-H4, found as a tetramer, and H2A-H2B dimers. Further studies will be needed to find out how the addition of the linker histone 1 (H1) protein causes the chromatin fiber to form a more compacted filament with a defined higher ordered structure. Covalent modifications such as acetylation, methylation, and phosphorylation are found on the histone tails allowing for regulatory contacts with the underlying DNA. Highly specific enzymes that converts these histone tail modifications have been identified and the list of them is getting longer by the day.

    The histone code hypothesis predicts that

    • distinct modifications of the histone tails will induce interaction affinities for chromation-associated proteins, and
    • modifications on the same or different histone tails may be interdependent and generate various combinations on any one nucleosome. Next,
    • (distinct qualities of higher order chromatin are largely dependent on the local concentration and combination of differentially modified nucleosomes. It is now thought that this allows a different “read-out” of the genetic information, such as gene activation versus gene silencing, or cell proliferation versus cell differentiation.

    Available experimental data link alterations in chromatin structure to cell cycle progression, DNA replication, DNA damage and repair, recombination and chromosome stability.

    combinatorial modifications Examples of combinatorial modifications in the histone amino acid termini that represent imprints for active or inactive chromatin. All four amino terminal ends of the core histones contain short basic patches that often comprise acetylation (Ac), phosphorylation (P), and methylation (Me) marks.

    Figure 7: Examples of combinatorial modifications in the histone amino acid termini representing imprints for active or inactive chromatin.

    The field of epigenetics has the potential to revolutionize the field of medical research and healthcare. It encompasses the study of nuclear components such as chromatin structure, including histone modifications, protein/DNA interactions, protein/RNA interactions, and how these factors influence gene function. It also includes the study of DNA methylation and the role that non-coding RNAs (ncRNAs) play in influencing DNA methylation patterns, chromatin structure and the regulation of gene expression. The polymerase chain reaction (PCR) can be used to study DNA methylation patterns, densities, and even the methylation status of individual cytosine residues. In addition, PCR methods have been developed to survey ncRNA expression and to identify regions of the genome where proteins and RNA interact or where certain functional histone marks are located.

    References

    Adams, J. (2008) Obesity, epigenetics, and gene regulation. Nature Education 1(1)

    Adrian Bird. Introduction Perceptions of epigenetics Nature 447, 396-398 (24 May 2007)

    Dolinoy, D. C., et al. Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development. Proceedings of the National Academy of Sciences 104, 13056–13061 (2007)

    Choudhuri S. Obesity, Epigenetics, and Gene Regulation By: Jill U. Adams, Ph.D. (Freelance Science Writer) © 2008 Nature Education

    Choudhuri S. From Waddington's epigenetic landscape to small noncoding RNA: some important milestones in the history of epigenetics research. Toxicol Mech Methods. 2011 May;21(4):252-74

    Duhl, D. M., et al. Neomorphic agouti mutations in obese yellow mice. Nature Genetics 8, 59–65 (1994).

    Andrew P Feinberg. Methylation meets genomics. Nature Genetics 27, 9 - 10 (2001)

    D. Haig. “The (Dual) Origin of Epigenetics” 2004, Cold Spring Harbor Symposia on Quantitative Biology Volume LXIX.

    Holliday R. The inheritance of epigenetic defects. Science. 1987 Oct 9;238 (4824):163-70.

    Holliday, Robin. “Epigenetics: A Historical Overview.” Epigenetics 1(2006): 76-80.

    Lu, D., et al. Agouti protein is an antagonist of the melanocyte-stimulating-hormone receptor. Nature 371, 799–802 (1994).

    Adele Murrell, Vardhman K. Rakyan and Stephan Beck; From genome to epigenome. Human Molecular Genetics, 2005, Vol. 14, Review Issue 1.

    Strahl BD, Allis CD. The language of covalent histone modifications. Nature. 2000 Jan 6;403(6765):41-5.

    Venter et al., 291 (5507): 1304-1351. The Sequence of the Human Genome Science 16 February 2001: Vol. 291 no. 5507 pp. 1304-1351.

    Robert A. Waterland and Randy L. Jirtle. Transposable Elements: Targets for Early Nutritional Effects on Epigenetic Gene Regulation. Mol. Cell. Biol. August 2003 vol. 23 no. 15 5293-5300

    Resources for Epigenetics

    Consortia and Initiatives

    The NIH Roadmap Epigenomics Program: A NIH Initiative to foster epigenomic research, develop comprehensive reference epigenome maps, and generate new technologies for comprehensive epigenomic analyses.

    http://nihroadmap.nih.gov/epigenomics/

    The Epigenome Network of Excellence: An EU-funded network of institutions and research groups

    http://www.epigenome-noe.net/WWW/index.php

    The Human Epigenome Projects: A public/private collaboration to catalogue Methylation Variable Positions (MVPs) in the human genome

    http://www.epigenome.org/

    NAME21: A German National Initiative to analyze DNA methylation Patterns of Genes on Chromosome 21

    http://biochem.jacobs-university.de/name21/

    Databases

    The Human Epigenome Atlas: The atlas includes human reference epigenomes and the results of their integrative and comparative analyses.

    http://www.genboree.org/epigenomeatlas/index.rhtml

    MethDB: A searchable database for DNA methylation and environmental epigenetic effects

    http://www.methdb.de/

    Human Histone Modification Database (HHMD): A searchable database of information from experimental data to facilitate understanding of histone modifications at a systematic level. The current release incorporates 43 location-specific histone modifications in human.

    http://bioinfo.hrbmu.edu.cn/hhmd

    NCBI Epigenomics: An online repository of epigenetic datasets

    http://www.ncbi.nlm.nih.gov/epigenomics/browse

    GeneImprint: A catalogue of imprinted genes

    http://www.geneimprint.com/site/genes-by-species

    Catalogue of Parent of Origin Effects: Searchable database of imprinted genes and related effects

    http://igc.otago.ac.nz/home.html

    Tools and Other Resources

    MethPrimer: Primer Design for Methylation PCR

    http://www.urogene.org/methprimer/index1.html

    MethBlast: A sequence similarity program that checks your primers for bisulfite converted DNA by blasting them against unmethylated and methylated genomic sequences of man, mouse and rat

    http://medgen.ugent.be/methBLAST

    Methylator: Methylator attempts to predict whether CpGs in a DNA sequence are likely to be methylated or not

    http://bio.dfci.harvard.edu/Methylator/

    RMAP: RMAP is a tool to map reads from the next-generation sequencing technology that supports bisulfite-treated reads mapping.

    http://rulai.cshl.edu/rmap/

    Chromatin Structure & Function: Information on chromatin biology, histones and epigenetics

    http://www.chromatin.us/chrom.html

    Epigenetic Station: A source for information, protocols, methods, techniques, products, vendors, kits, assays, analysis, bioinformatics and databases on Epigenetics

    http://epigeneticstation.com/

    SMART :http://smart.embl-heidelberg.de/


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  • 04/06/14--00:00: Retro Inverso Peptides
  •  Retro-inverso peptides are linear peptides whose amino acid sequence is reversed and the α-center chirality of the amino acid subunits is inverted as well. Usually, these types of peptides are designed by including D-amino acids in the reverse sequence to help maintain side chain topology similar to that of the original L-amino acid peptide and make them more resistant to proteolytic degradation. Other reported synonyms for these peptides in the scientific literature are: Retro-Inverso Peptides, All-D-Retro Peptides, Retro-Enantio Peptides, Retro-Inverso Analogs, Retro-Inverso Analogues, Retro-Inverso Derivatives, and Retro-Inverso Isomers. D-amino acids represent conformational mirror images of natural L-amino acids occurring in natural proteins present in biological systems. Peptides that contain D-amino acids have advantages over peptides that just contain L-amino acids. In general, these types of peptides are less susceptible to proteolytic degradation and have a longer effective time when used as pharmaceuticals. Furthermore, the insertion of D-amino acids in selected sequence regions as sequence blocks containing only D-amino acids or in-between L-amino acids allows the design of peptide based drugs that are bioactive and possess increased bioavailability in addition to being resistant to proteolysis. Furthermore, if properly designed, retro-inverso peptides can have binding characteristics similar to L-peptides. Retro-inverso peptides are useful candidates for the study of protein-protein interactions by designing peptidomimetics that mimic the shape of peptide epitopes, protein-protein, or protein-peptide interfases. Retro-inverso-peptides are attractive alternatives to L-peptides used as pharmaceuticals. These of peptide have been reported to elicit lower immunogenic responses compared to L-peptides. The availability of modern chemical synthesis methods now allows the routine synthesis of these types of peptides.

     
     

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    Antibody-drug conjugates, also called ADCs, are site-specifically modified antibodies that are designed as a targeted therapy for the treatment of cancer and are considered as a new class of highly selective, potent drugs. These complex molecules are made up of an antibody that is connected or conjugated to a cytotoxic drug via a stable chemical linker utilizing optimized coupling chemistries for their production. Sometimes a whole monoclonal antibody (mAb), a reengineered mAb or an antibody fragment, such as a single-chain variable fragment (scFv), is linked via a stable chemical linker with labile bonds, to a biological active drug. Therefore, ADCs are pro-drugs with drugs covalently bound to monoclonal antibodies in which the antibody serves to deliver the drug to the intended site of action by binding to antigens at the site of action, for example to a tumor. To achieve this, the binding of the antibody has to be very specific to avoid off-target effects or binding. In addition the drug needs to be slowly released in the target cells. This type of drug is also called a toxophor and the total drug load has been called the “payload”. Since the total amount of conjugated drug or payload is limited, the toxophor has to exhibit a high potency. In addition, the drug to antibody ratio („DAR“) is critical for antibody stability. To be useful as a therapeutic drug the pharmacokinetics, abbreviated as PK, of the ADC as well as the active drug has to be determined to evaluate efficacy, toxicity and the response to the drug exposure.

    The use of biological therapies for the treatment of cancer is now a fast growing field. Unfortunately, the use of naked antibodies that show clinical efficacy has proven to be limited. To enhance the therapeutic potential of an antibody the conjugation to a small drug molecule has been heavily investigated in recent years. This approach is expected to combine the benefits of highly potent drugs with selective binders of specific tumor antigens. In addition, the next generation of ADCs are designed to utilize site-specific coupling chemistries using protein engineering with the aim to maintain the specificity and potency but to also improve the half-life, the homogeneity of drug loading, safety and quality of the therapeutic molecule. However, designing an ADC is a complex undertaking that requires the thoughtful combination of a selected antibody with a chemical linker group and a drug that targets a defined cancer indication. Lessons learned from the first-generation antibody-drug conjugates resulted in the improvement of the technology that now guides the design of new improved compounds, some of which are now in clinical trials. Intense research is ongoing to help improve ADCs further. Various linkers that show good in vitro stability and have a positive effect on ADC PK and in vivo efficacy are now designed and investigated.

    As of 2013 a total of 27 ADC were undergoing clinical trials in both hematological malignancies and solid tumor indications. One ADC called T-DM1 or trastuzumab emtansine showed very promising results in phase III for the treatment of HER2-positive refractory/relapsed metastatic breast cancer. Other compounds currently in clinical trials are CMC-544, SAR3419, CDX-011, PSMA-ADC, BT-062, and IMGN901, targeting varied antigens. In additions, a search performed in April 2014 at “ClinicalTrials.gov” for the term “antibody-drug-conjugates” showed that 213 clinical trials were ongoing and some of them are recruiting.

    Since the ADCs are exposed to different conditions during their journey from the blood vessels to the molecular target in the tumor tissue the mode of action of these drugs at the cellular or molecular level is complex. During circulation in the plasma the ADC must behave like a natural antibody and the linker must be stable. During antigen binding it is necessary that the conjugated antibody retains high immunoaffinity and the attached drug compound must not disturb this. During internalization a sufficient intracellular concentration of the drug must be achieved. After internalization during drug release the ADC has to efficiently release the original cytotoxic drug in its active form in the tumor. And finally, the inherent potency of the released drug must be sufficient to kill the tumor cells. These criteria demand that the ADCs are properly designed to achieve optimal effects.

    References and Links

    1. ADC Review
    2. Laurent Ducry and Bernhard Stump; Antibody-Drug Conjugates: Linking Cytotoxic Payloads to Monoclonal Antibodies. Bioconjugate Chem. 2010, 21, 5–13.
    3. Makign hot ADC
    4. Sassoon I, Blanc V.; Antibody-drug conjugate (ADC) clinical pipeline: a review. Methods Mol Biol. 2013;1045:1-27. doi: 10.1007/978-1-62703-541-5_1.

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  • 04/15/14--00:00: Protein Moonlighting
  • Protein moonlighting or gene sharing refers to the phenomenon by which proteins perform multiple functions in an organism. A big surprise as a result of sequencing the human genome was the discovery that the human genome appears to code for far fewer proteins than what was predicted. Successive research and investigating the biochemistry of proteins that make up the so called proteome revealed that this could be partially explained by alternative splicing of mRNA transcripts and/or the 'reusing' of proteins for other additional purposes or functions. For example, it was known for quite some time that several soluble enzymes can perform a second function, for example, structural proteins found in the lens of the eye called crystallins. Other proteins that showed a similar behavior are proteins that bind DNA or RNA and are involved in the regulation, translation or transcription of genes. However, more precisely "moonlighting" refers to a single protein that has multiple functions not because of gene fusions, splice variants or multiple proteolytic fragments. Many proteins are now known to moonlight. These include receptor- , trans-membrane channel-, chaperone-, ribosomal proteins, the proteins Clf1p, Sug1/Rpt6 and Sug2/Rpt4, and, potentially, and potentially many others. For example, Clf1p is an essential, highly conserved protein found in S. cerevisiae that has been implicated in pre-mRNA splicing. Sug1/Rpt6, a component of the 19S complex, belongs to the six highly conserved ATPases of the AAA class in the 19S proteasome regulatory particle and a molecular target of RIP-1. RIP-1 refers to the cell death protein "Receptor-interacting protein 1" which is a serine/threonine-protein kinase. The term AAA or AAA+ is an abbreviation for 'ATPases Associated with diverse cellular Activities'. These proteins share a common conserved module or motif of approximately 230 amino acid residues and belong to a functionally diverse protein family, the AAA+ superfamily of ring-shaped P-loop RNA nucleoside triphosphatases (NTPases). AAA ATPases are usually associated with various cellular activities where they play important roles including proteolysis, protein folding, membrane trafficking, cytoskeletal regulation, organelle biogenesis, DNA replication, and intracellular motility. In the past, the identification of moonlighting proteins has been done by chance since there is no clear procedure available for the identification of the secondary functions of a protein. However, various methods have been used to determine a protein's multiple function such as the use of co-precipitation methods, including immune-co-precipitation. The use of these methods in combination with mass spectrometry based methods may now allow for the successful identification and characterization of new members of moonlighting proteins. Furthermore, it is thought that moonlighting proteins can switch functions due to a change in cellular localization, expression in a novel cell type, oligomeric state, and cellular concentration of a ligand, substrate, cofactor or product. It appears that protein moonlighting may occur widely in nature. Unfortunately, in the case of a mutation that causes a disease, the existence of moonlighting proteins could make the development of treatments for diseases caused by this mutation more difficult. In addition, currently there is no general straightforward method available which allows for the identification of moonlighting proteins. More research will need to be done.


    Reference

    Constance J. Jeffery; Moonlighting proteins: old proteins learning new tricks. TRENDS in Genetics Vol.19 No.8 August 2003, 415-417.

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  • 04/25/14--00:00: Collagen Mimetic Peptides
  • By: Klaus D. Linse

    Collagen mimetic peptides, or CMPs, are typically made of 30 or fewer amino acids. These types of peptides are usually composed of multiple helix promoting peptide trimers. Collagens are integral structural proteins which are among the most diverse and abundant proteins found in the animal kingdom where they play key functional roles in cellular modulation. Therefore these proteins have attracted scientists in the research fields of supramolecular chemistry, biomedical and materials science in recent years as a guide for the design of unique synthetic biomolecules. In the last three decades CD4 α-turn mimetic peptides that inhibit human immunodeficiency virus envelope glycoprotein gpl20 binding and infection of human lymphocytes have been designed and synthesized. In addition the design of similar peptide mimics has blossomed as well. Collagen mimetic peptides were initially developed and used by biochemists for the investigation and elucidation of the structures and stability of natural collagens. Biologists and polymer chemists followed soon to produce nanostructured fibrous scaffolds using collagen mimetic peptides as the building blocks. The design of CMPs is based on ProProGly and ProHypGly trimer sequence motifs. The best characterized CMPs to date contain the collagen-like triple-helical structure within their peptide sequence and show reversible melting characteristics that are well documented in the literature. Over the years modern synthesis methods have been developed employing techniques such as ligation chemistries based on activated esters, click chemistry, carbodiimide chemistry or other ligation chemistries. These methods now provide synthetic scientists versatile strategies to prepare collagen-polymer conjugates. Furthermore, researchers observed that these collagen mimetic peptide conjugates can spontaneously assemble when stimulated accordingly. Development of engineered tissue and organ replacement therapies has increased in recent years, promoting a emand for new approaches to immobilize components derived from outside cells or tissue to natural collagen.

     

    These peptide mimics have similar behavior as amphiphilic peptides that are known to form defined nanostructures such as molecular wires, well defined nanotubes as well as nanovesicles. Amphiphilic peptides have been used as scaffolds for the synthesis of defined nanometer structures in recent years and studies of biological systems on the molecular level in the 20th century revealed that molecular self-assembly is a fundamental process in all living systems.

    Wang et al. in 2004 developed an alternative to the conventional “covalent” modification methods called a “physical” modification technique that is based on collagen’s native ability to associate into a triple-helical molecular architecture. Chemical coupling of synthetic moieties to amino acid side chains such as lysines (K, Lys) or glutamines (E, Glu) is a routinely used technique for such purposes. Unfortunately, these types of coupling reactions are difficult to control when used on large proteins and generally are not easy to control when modifying integrated collagen scaffolds that contain live cells and tissues. To circumvent this the research group synthesized collagen mimetic peptides containing the sequence -(Pro-Hyp-Gly)- multiple times. The scientists report that these peptides exhibit a strong affinity to both native and gelatinized type I collagen under controlled thermal conditions. Furthermore, they show that the cell adhesion characteristics of collagen can be readily altered by applying a poly (ethylene glycol)-CMP conjugate to a prefabricated collagen film. The next table shows the melting behavior of selected peptides.

    Table 1:   Melting Transition Temperatures of Collagen Mimetic Peptide Derivatives Determined by Circular Dichroism Spectroscopya  (Source: Wang et al., 2005).

       

    Compound

    Sequence

    Tm (°C)

    1

    -(ProHypGly)10-

    69

    2

    5CF-Gly3-(ProHypGly)10-

    75

    3b

     

    5CF-Gly3-randomPro10Hyp10Gly10-

     

    4

    5CF-Gly3-(ProHypGly)6-

    25

    a  Measured in 57.5 µM acetic acid solution.

    b  5CF-GGGGPPPHPHGPGGG PPHPPHGPHGPPHPGPHPHPGGPHPHPP, (PH:Hyp).

    c mPEG2000, CH3O-(CH2-CH2-O)n-OH, 2250 Da.

     

    The researchers demonstrated the binding of the synthetic CMP to natural acid soluble, bovine type I collagen or denatured gelatin collagen by treating collagen films with solutions of a fluorescently labeled CMP. Results from rinsing the treated collagen films and measuring the fluorescence intensity of the exposed film suggested that 5-carboxy fluorescein (5CF)-labeled-Gly3-(ProHypGly)10- tightly attached to partially denatured collagen when it is introduced as a single strand. The ability of the peptide to assemble into a triple helix appears to be essential for the attachment to happen. The researchers argue that the ability to control the organization of cells in collagen matrices may provide a new pathway for engineered tissues and that the affinity between the CMP and collagen could be used to immobilize therapeutic drugs to collagens in the living tissues and biomaterials that incorporate natural collagens.

     

    In 2007 Rele et al. designed and synthesized collagen-mimetic triple helix promoting peptides that self-assembled into a fibrous structure with well-defined periodicity as visualized by transmission electron microscopy (TEM). The researchers used a Xaa-Yaa-Gly triad sequence to create sequence specific peptides containing three different Xaa-Yaa-Gly domains, including a central core of Pro-Hyp-Gly repeat sequences flanked by distinct sets of peptide repeats, containing either negatively (Glu) or positively (Arg) charged amino acid residues. The Pro-Hyp-Gly peptide sequence was reported to form the structurally critical hydrophobic core of the assembly, responsible for maintaining the thermodynamic stability of the collagen triple-helical structure. Furthermore, the researchers reasoned that the synthesis of collagen-mimetic triple helix peptide protomers (THPs) that display the capacity to form triple helices with improved stability and that exhibit a propensity to form linear assemblies through a process of axially oriented alignment will prove to have a number of important practical applications in the design of novel biomaterials. These types of material may lend themselves for the development of collagen-based biomaterials for wound healing.

     

    In 2008 Cejas et al. synthesized collagen model peptides that form triple helices and self-assemble into supra-molecular fibrils exhibiting collagen-like biological activity without the need for preorganizing the peptide chains by covalent linkages. The researchers accomplished this by placing aromatic groups on the ends of a representative 30-mer CMP, (GPO)10, by using L-phenylalanine and L-pentafluorophenylalanine  in 32-mer. The use of atomic force microscopy topographical imaging indicated that some of these peptides self-organized into microfibrillar species. In addition, two peptides, 1a and 1b, where reported to induce the aggregation of human blood platelets with a potency similar to type I collagen.

     

    Su et al. in 2010 demonstrated that treatment with the apoA-I mimetic peptides, L-4F, D-4F (the peptide Ac-D-W-F-K-A-F-Y-D-K-VA-E-K-F-K-E-A-F-NH2 synthesized from all L- or all D-amino acids, respectively), or L-5F (Ac-D-W-L-K-A-F-Y-D-K-V-F-EK-F-K-E-F-F-NH2, synthesized from all L-amino acids) decreases tumor burden in mice injected with ID8 cells.

     

    Yu et al. in 20011 reviewed progress made in the field of collagen mimetic peptides that are useful for the design and synthesis engineered collagen-like materials for potential biomedical use. The scientists report that the collagen triple helix has become a promising structural motif for engineering self-assembled, hierarchical constructs similar to natural tissue scaffolds. Further, they discuss various CMPs and collagen-like proteins that mimic either structural or functional characteristics of natural collagens. This paper provides helpful information to bioengineers and biomaterials scientists interested in collagen engineering.

     

    Li et al. in 2012 reported the synthesis and use of collagen mimetic peptides (CMPs) that can be phototriggered to fold into triple helix and bind to collagens denatured by heat or by matrix metalloproteinase (MMP) digestion.  The peptide binding assays that were used by this research group indicated that the binding is primarily driven by stereo-selective triple-helical hybridization between monomeric CMPs of high triple-helical propensity and denatured collagen strands. Furthermore the scientists showed that photo-triggered hybridization allows specific staining of collagen chains in protein gels as well as photo-patterning of collagen and gelatin substrates. Their in vivo experiments demonstrated that systemically delivered CMPs can bind to collagens in bones, and in articular cartilages and tumors characterized by high MMP activity. They further showed that CMP-based probes can detect abnormal bone growth activity in a mouse model of Marfan syndrome. This approach allowed the researcher targeting the microenvironment of abnormal tissues.

    He et al. in 2013 reviewed modern synthesis methods that were developed for the synthesis of collagen mimetic peptide conjugates used in polymer science. These methods employ particular ligation chemistries basing on activated ester, click chemistry, carbodiimide chemistry or other ligation chemistries allowing the preparation of collagen-polymer conjugates. Furthermore, the researchers point out that these conjugates made with collagen mimetic peptides as the building blocks show exciting stimuli responsive or spontaneously assembly behavior.

    All these findings have let researchers in the tissue engineering and biomedical field now to speculate that the ability to control the organization of cells in collagen matrices may provide new pathways to engineer new types of tissues. Furthermore, the affinity between the CMPs and collagen could be used to immobilize therapeutic drugs to collagens in living tissues and biomaterials that incorporate natural collagens.

    To conclude, highly helical peptides can now be made using a variety of peptide sequences. These types of peptide mimics may have many applications in experimental biology, biomedicine and tissue engineering. Since many peptides can retain ligand-binding properties of proteins from which they are derived they may act as inhibitors of antigen-antibody reactions or of hormone-receptor interactions, which would make them good starting molecules for the design of new types of biomaterials.

     

    References

    Mabel A. Cejas, William A. Kinney, Cailin Chen, Jeremy G. Vinter, Harold R. Almond, Jr., Karin M. Balss, Cynthia A. Maryanoff, Ute Schmidt, Michael Breslav, Andrew Mahan, Eilyn Lacy, and Bruce E. Maryanoff; Thrombogenic collagen-mimetic peptides: Self-assembly of triple helix-based fibrils driven by hydrophobic interactions. PNAS June 24, 2008 vol. 105 no. 25, 8513–8518.

     

    SHAOXING CHEN, R. ALAN CHRUSCIEL, HIROSHI NAKANISHIt, APICHAYA RAKTABUTR,

    MICHAEL E. JOHNSON, ALICE SATO, DAVID WEINER, JIM HOXIE, HORACIO URI SARAGOVI, MARK I. GREENE, AND MICHAEL KAHN; Design and synthesis of a CD4 a-turn mimetic that inhibits human immunodeficiency virus envelope glycoprotein gpl20 binding and infection of human lymphocytes. Proc. Natd. Acad. Sci. USA Vol. 89, pp. 5872-5876, July 1992.

     

    Lirong He, Patrick Theato,  Collagen and collagen mimetic peptide conjugates in polymer science. European Polymer Journal, Volume 49, Issue 10, October 2013, Pages 2986–2997.

     

    Yang Li, Catherine A. Foss, Daniel D. Summerfield, Jefferson J. Doyle, Collin M. Torok, Harry C. Dietz, Martin G. Pomper, and S. Michael Yu; Targeting collagen strands by photo-triggered triple-helix hybridization. 10.1073/pnas.1209721109 PNAS September 11, 2012 vol. 109 no. 37 14767-14772.

    Shyam Rele, Yuhua Song, Robert P. Apkarian, Zheng Qu, Vincent P. Conticello, and Elliot L. Chaikof; D-Periodic Collagen-Mimetic Microfibers. J. AM. CHEM. SOC. 2007, 129, 14780-14787.

     

    Feng Su, Kathy R. Kozaka, Satoshi Imaizumib, Feng Gaoa, Malaika W. Amneusa, Victor Grijalvab, Carey Nga, Alan Wagnerb, Greg Houghb, Gina Farias-Eisnerb, G. M. Anantharamaiahc, Brian J. Van Lentenb, Mohamad Navabb, Alan M. Fogelmanb, Srinivasa T. Reddya, and Robin Farias-Eisnera; Apolipoprotein A-I (apoA-I) and apoA-I mimetic peptides inhibit tumor development in a mouse model of ovarian cancer. PNAS | November 16, 2010 | vol. 107 | no. 46 | 19997–20002.

     

    Wang AY, Mo X, Chen CS, Yu SM; Facile modification of collagen directed by collagen mimetic peptides. J Am Chem Soc. 2005 Mar 30;127(12):4130-1.

    S. Michael Yu, Yang Li and  Daniel Kim; Collagen mimetic peptides: progress towards functional applications. Soft Matter, 2011,7, 7927-7938.


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  • 05/07/14--00:00: Gold Nanoparticle Properties
  • Gold nanoparticles (colloidial gold) have been extensively used for applications both in biology (e.g. bio-imaging) and technology (e.g. photonics) due their unique optical properties. These properties are conferred by the interaction of light with electrons on the gold nanoparticle surface. At a specific wavelength (frequency) of light, collective oscillation of electrons on the gold nanoparticle surface cause a phenomenon called surface plasmon resonance resulting in strong extinction of light. The particular wavelength, or frequency, of light where this occurs is strongly dependant on the gold nanoparticle size, shape, surface and agglomeration state as described in more detail below.
     

    Gold Nanoparticle Size

    The influence of gold nanoparticle size on the surface plasmon resonance is illustrated in figure 1 below where the absorption maximum (lambda max) increases from 520nm to 570nm for 20nm and 100nm spherical gold nanoparticles, respectively. Particles with sizes above 100nm have broader peaks spanning into the 600nm range due to the presence of both transversal and longitudinal surface plasmon resonances. In comparison, gold nanoparticles with diameters below 2nm do not exhibit surface plasmon resonance.

    The difference in extinction between different sized gold nanoparticles can conveniently be utilized for multiplexing.

    gold nanoparticle absorbance

    Figure 1. Gold nanoparticle size dependant surface plasmon resonance. Note the red-shift of the absorption maximum as the gold nanoparticle size increases.

    Gold Nanoparticle Shape

    A major determinant of the optical properties of gold nanoparticles is their shape. By synthesizing gold nanoparticles of different shapes, the surface plasmon resonance can easily be tuned to give absorption maxima from around 500nm into the near-infrared part of the spectrum. As an example, spherical collodial gold have absorbance maxima between 515-570nm as described above, while irregular shaped particles such as gold nanorods, and urchin shaped gold nanoparticles (also called gold nanostars) have absorption maximum in the near-infrared region of the spectra, figure 2. For custom synthesis of gold nanoparticles with irregular shapes please contact us.

    The difference in absorption properties between spherical and irregular-shaped gold nanoparticles of the same average size is caused by an anisotropic (uneven) distribution of the surface electron layers.

    spherical vs irregular shaped gold nanoparticles absorbance

    Figure 2. (Top) Gold nanoparticle shape dependant localized surface plasmon resonance as indicated by the visual appearance and UV-VIS spectra of spherical (A), and urchin-shaped (B) gold nanoparticles (gold nanostars). (Bottom) Absorbance spectra for gold nanorods with three different aspect ratios. Note the presence of two absorption peaks, which are caused by both transversal and longitudinal surface plasmon resonances.

    Urchin shaped (spiky) gold nanoparticles (gold nanostars) are preferable over spherical particles in in vivo based applications due to a reduced background, and higher penetration of near-infrared light through biological tissues. Also, irregular-shaped gold nanoparticles give higher signal in Surface-Enhanced Raman Spectroscopy (SERS) due to enhancement of the electromagnetic field on the surface caused by the irregular shaped particles. In comparison, spherical particles are ideal for use in application such as immunogold dot-blot protocols (see figure 3 below) and lateral flow rapid tests.

    immuno dot-blot noble metal nanoparticle protein conjugates

    Figure 3. Immuno-dot blot assay illustrating the difference in appearance (color) for three different types of noble metal protein conjugates varying in shape and composition.

    Gold Nanoparticle Aggregation State

    As mentioned above, the aggregation state of gold nanoparticles have an effect on their optical properties. This fact can be used to monitor gold nanoparticle stability, both over time, and upon addition of salt-containing buffers, which at high enough concentrations cause particle aggregation, figure 4. The red-shift in absorption maximum caused by aggregation, or particles in close proximity, have successfully been utilized in many assays as a detection mechanism.

    gold nanoparticles nacl

    Figure 4. Visual appearance and UV-VIS spectra of monodisperse (A) and sodium chloride (NaCl) induced agglomeration (B) of 15nm gold nanoparticles.
     

    Gold Nanoparticles Optical and Physical Specifications at Different Sizes







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    Storage:

    Store product away from direct sunlight at 4-25°C. Lower temperature prolongs the shelf life of the product. Do NOT freeze. If frozen, the gold nanoparticles will irreversibly aggregate. This is indicated by a change in color of the solution, see figure below. When stored as specified the colloidal gold is stable for at least 1 year.

    appearance of frozen gold nanoparticles


    Handling:

    When stored for a long period of time the gold nanoparticles might sediment at the bottom of the flask, which is especially true for larger particle sizes. Prior to use, re-suspend the sedimented particles by swirling until a homogenous solution is obtained.

    To maintain optimal performance, and stability of the colloidal gold, care should be taken to use clean storage containers if using other than supplied with the product.
     

    Washing:

    Although it is not always necessary to wash the gold nanoparticles prior to use, some applications might require additional washing procedures. The easiest way to remove possible contaminants in the nanoparticles solution is by centrifugation. Centrifugation force is dependant on size of the gold nanoparticles and should be adjusted according to table I for optimal performance.

    Note:
    Since non-functionalized gold nanoparticles are sensitive to salt containing buffers, re-suspension should always be performed in ultra-pure water to prevent irreversible aggregation. Irreversible aggregation is characterized by a clear to bluish solution upon the addition of salt.

    Procedure:

    1. Place aliquot of colloidal gold in appropriate centrifuge tube.
    2. Centrifuge the gold nanoparticles for 30 minutes using the appropriate G force depending on size of the gold nanoparticles, see Table I.
    3. Remove supernatant and re-suspend in appropriate volume of ultra-pure water.
    4. Vortex to re-disperse particles.
    5. Repeat as necessary

    Table I. Appropriate G forces for centrifugation of gold nanoparticles. Note that recommended conditions are for a volume of 1ml and centrifugation using a microcentrifuge, except for 5nm gold nanoparticles that requires an ultracentrifuge.
     

    Size (nm) Speed (g) Time (min)
    5 100,000 30
    10 17,000 60 (~50% recovery)
    15 17,000 30
    20 6,500 30
    30 4,500 30
    40 2,500 30
    50 2,000 30
    60 1,125 30
    80 600 30
    100 400 30
    150 180 30
    200 100 30

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    Gold nanoparticle conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy. Stable gold nanoparticle conjugates can easily be prepared by passive adsorption due to electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold. This process is maximally achieved at a pH close to the pI of the protein to be conjugated. An important parameter to consider when preparing gold nanoparticle conjugates is the amount of protein bound to the gold colloid. If too little protein is adsorbed to the gold surface, aggregation occurs upon addition of electrolytes present in standard buffers. A titration is therefore performed to determine at which protein concentration saturation and colloidal stability is reached.


    Materials and Equipment Required:

    Standard Gold nanoparticles
    10% NaCl
    10% PEG (5,000-20,000Da)
    Phosphate Buffered Saline, PBS
    Protein to be conjugated
    UV-VIS Spectrophotometer
     

    Titration procedure to find amount of protein needed to saturate and stabilize the gold colloid:

    1. Transfer 250μl of resuspended gold nanoparticles and place in 1.5ml Eppendorf tubes.
    2. Adjust pH of the gold colloid to match the isoelectric point (pI) of the protein to be conjugated.
    3. Add between 0 and 1mg of protein in 25μl to the gold nanoparticles while mixing to titrate the amount needed to saturate the gold surface.
    4. Incubate for 2-3 minutes at room temperature.
    5. Add 250μl of 10% NaCl solution.
    6. Observe color change and determine at which protein concentration the gold nanoparticle surface is saturated and no aggregation occurs upon addition of 10% NaCl. This can be observed by an increase in absorbance at 580nm compared to the control.

    Note: The amount of protein needed to saturate the gold colloid can also be determined by agarose gel electrophoresis due to the change in charge upon binding of the protein.

    Preparation of the gold nanoparticle conjugate:

    1. Transfer amount of gold nanoparticles needed for your application from the stock to a new tube.
    2. Add protein amount as determined above plus an additional 10%.
    3. Incubate for 30 minutes at room temperature while stirring.
    4. Centrifuge the solution for 30 minutes at the appropriate speed for the gold nanoparticle size used.
    5. Resuspend the pellet in PBS supplemented with 0.1% BSA or 1% PEG.
    6. Store at 4°C until use.


    References:

    Thobhani, S., Atree, S., Boyd, R., Kumarswami, N., Noble, J., Szymanski, M., Porter, R.A. (2010)
    Bioconjugation and characterization of gold colloid-labelled proteins Journal of Immunological Methods 356, 60-69


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    Due to the relative ease of preparing antibody gold conjugates, and straightforward detection of gold nanoparticles by naked eye without prior development procedures, these probes has found great use in rapid tests such as lateral flow, western blot and dot-blot assays.

    When membranes of blotted proteins are probed with a secondary gold conjugate, the presence of a target protein is indicated with a red color upon binding of the gold conjugate.

    When combined with silver enhancement (i.e. deposition of silver onto bound gold colloid turning the spot dark in color) sensitivity in western blot and dot-blot applications rivals that of colorimetric detection methods. In addition, secondary gold probes adapt well to standard western blot protocols and little changes are necessary to your current detection scheme.


    Standard Immunogold Dot-Blot Protocol

    (Adapted from Moeremans et al. [1])
    1. Spot one microlitre drops of a serial dilution of your protein (100-0.1ng) in PBS supplemented with 50ug/ml of BSA on nitrocellulose or PVDF membrane.
    2. Let protein drops dry into the membrane.
    3. Block with Membrane Blocking Solution for 30 minutes at room temperature.
    4. Inubate with primary antibody for 2 hours at room temperature.
    5. Wash membrane 3x5 minutes with membrane blocking solution.
    6. Incubate for 2 hours (or longer for increased sensitivity) with secondary gold conjugate diluted 1:10-1:200 times with membrane blocking solution for preparation.
    7. Wash 3x5 minutes as above.
    8. Dry membrane and record data.
    9. (OPTIONAL) Proceed with silver enhancement to improve sensitivity
    gold and silver conjugate dot-blot with or without silver enhancement comparison between a standard gold conjugate and silver conjugate and gold nanourchin conjugates

    Figure 1. Example dot-blot assay for streptavidin gold conjugate (top left) and streptavidin silver conjugate (top right) before and after enhancement using silver enhancement kit for membranes. Bottom picture illustrates and highlights the difference in appearance (color) of 50nm anti-human IgG noble metal nanoparticle conjugates prepared using NHS-activated gold nanoparticles, NHS-activated gold nanourchins and NHS-activated silver nanoparticles, respectively.
     

    References

    1. M. Moeremans, et al.Journal of Immunological Methods, 1984, 74, 353


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  • 05/07/14--00:00: Lateral Flow Immunoassays
  • While rapid assay methods have made a major impact on a variety of diagnostic testing over the last twenty years only a handful of development can make the claim to have taken testing out of the laboratory.

    One that can, and is in widespread use as a result, is the lateral flow immunoassay test, also known as the immunochromatography assay, or strip test. Like many of the best ideas, lateral flow immunoassays take clever and sophisticated technology and turn it into something so simple to operate that alsmost anyone can use it.
     

    The Technology

    The basic technology that underlies lateral flow immunoassays was first described in the 1960s, but the first real commercial application was Unipath’s Clearview home pregnancy test launched in 1988. Since then, this technology has been employed to develop a wide and ever-growing range of assays for clinical, veterinary, agricultural, food industry, bio-defence and environmental applications.

    Strip assays are extremely versatile and are available for an enormous range of analytes from blood proteins to mycotoxins and from viral pathogens to bacterial toxins. Assays has even been developed for wine producers to assess the amount of botrytis rot in newly harvested grapes as well as for use in the clinical lab identifying cardiac chemistries. This shows the vast range that this technology can be applied too.

    Lateral flow immunoassays are essentially immunoassays adapted to operate along a single axis to suit the test strip format. There are a number of variations of the technology that have been developed into commercial products, but they all operate using the same basic principle.
     

    How does a lateral flow assay work?

    A typical test strip consist of the following components:

    1. Sample pad - an adsorbent pad onto which the test sample is applied.
    2. Conjugate or reagent pad – this contains antibodies specific to the target analyte conjugated to coloured particles (usually colloidal gold particles, or latex microspheres).
    3. Reaction membrane – typically a hydrophobic nitrocellulose or cellulose acetate membrane onto which anti-target analyte antibodies are immobilised in a line that crosses the membrane to act as a capture zone or test line (a control zone will also be present, containing antibodies specific for the conjugate antibodies).
    4. Wick or waste reservoir – a further absorbent pad designed to draw the sample across the reaction membrane by capillary action and collect it.

    The components of the strip are usually fixed to an inert backing material and may be presented in a simple dipstick format or within a plastic casing with a sample port and reaction window showing the capture and control zones.

    Image of lateral flow setup

    We provide a full product line of gold nanoparticles (colloidal gold) for use in a variety of lateral flow assays. A diverse product line of different type of nanoparticles offers you products with a narrow size distribution (CV of less than 12%), exceptional adsorption and conjugation properties and with greater than 95% spherical particles. In addition, batch to batch variability is extremely low (+/- 2nm), which assures that customer will always end up with a product within the specified size range that you ordered.

    The high shape uniformity of colloidal gold will minimize the variability within your assay by e.g. allowing control over the available surface area while absorbing or covalently conjugating proteins to gold nanoparticles. It will also ensure a more uniform flow rate across your membrane for improved reproducibility and overall results.


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    Gold nanoparticles have many applications in biology and medicine. Gold nanoparticles are used as biosensors, cellular probes, drug delivery vehicles, or as optical contrast agents (1). To use gold nanoparticles for these specific applications, the ability to modify the surface is extremely important. A modification of the surface with polymers such as polyethylene glycol (PEG) and polyethylene oxides reduce non-specific binding, small molecules such as biotin and peptides and proteins can provide them specificity toward cellular targets in vitro and in vivo, and DNA enables them to be used for genetic detection. A key strategy to modifying the surface of the gold nanoparticles is with molecules that contain sulfur, nitrogen, or oxygen atoms as they have high binding affinity to gold atoms. As a result, thiolated-DNA and proteins adsorb onto the surface of the gold nanoparticles relatively easily. The design of gold nanoparticles allows for the use of either adsorption to coat polymers or bio-recognition molecules on the surface, by covalent conjugation, or by using biological interactions (e.g., streptavidin-biotin). This provides greater versatility in the design of you gold nanoparticle system. This technical note provides a brief description of the current modification strategies and some of the emerging biomedical applications of these materials.


    Surface Modification Strategies

    Pegylated gold nanoparticles

    PEG is a polyether molecule and is typically described by the molecular weight and whether they are linear, branched, star, or combed-shaped. PEG molecules can also be functionalized with thiols, amines, carboxylic acids, or alcohols. PEG molecules are coated onto gold nanoparticles by a sulfur-gold atom bond. Interestingly, coating of a dense layer of PEG onto gold nanoparticles has shown to reduce non-specific binding of proteins (2). It was recently shown that gold nanoparticles with greater than 0.96 PEG/nm2 is required to reduce non-specific binding and to inhibit their uptake into macrophage cells. Using these results, PEGylated gold nanoparticles were designed with the lowest non-specific cellular uptake. This is very important when using gold nanoparticles in biology where the particles are programmed to target specific molecules or cellular receptors. Non-specific protein binding can affect the specificity. In addition, the gold nanoparticle-PEG system contains protruding carboxylic acids or amines that allows other molecules to be conjugated to the surface by using the coupling agent 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, better known as EDC. In this reaction, the gold nanoparticles is incubated with EDC and the biological molecule of interest for 2 hrs and then purified by centrifugation to remove excess biological molecules. These gold nanoparticle conjugates are then ready to be used for biological purposes.

    Gold nanoparticles coated with bio-recognition molecules

    Gold nanoparticles cannot recognize specific biological targets without surface modification. It is well known that single-stranded DNA’s called oligonucleotides can recognize a complementary sequence, antibodies recognize antigens, and peptides recognize antibodies. By coating the surface of gold nanoparticles with oligonucleotides, antibodies, peptides, or other bio-recognition molecules, one is then able to recognize specific targets in solution, in western blots (see figure 1), on/in cells, or in tissues in animals. Basically, these molecules provide gold nanoparticle with a biological function. Typically these bio-recognition molecules are coated onto the surface via direct adsorption or by covalent conjugation to the gold nanoparticle surface containing carboxylic acid or amine functional groups.

    antigen detection gold conjugate dot-blot

    Figure 1. Immuno-dot blot assay for detection of human IgG antibodies using gold nanoparticles, silver nanoparticles and gold nanourchins ("spiky gold") coated with bio-recognition molecules. Note how the difference in appearance (color) of the dots can be achieved using different types of noble metal protein conjugates varying in either shape or composition.

    Heterogeneous surface design

    Engineering a gold nanoparticle surface that contains PEG and bio-recognition molecules is ideal for their application. The PEG molecule prevents non-specific binding while the bio-recognition molecule provides biological specificity. Gold nanoparticle products enables researchers to design their nanoparticles in this manner, for optimum biological use.

    Applications

    Gold nanoparticles coated with PEG or/and bio-recognition molecules (BRM) has many applications, e.g. biosensors, cellular probes, drug delivery vehicles, or as optical contrast agents. Below, are some specific examples of how to use gold nanoparticle conjugates.

    DNA sensors

    Single stranded DNA-coated onto gold nanoparticles can be used for detection of genetic material (3). In this application, single stranded oligonucleotide-coated gold nanoparticles are incubated with a DNA fragment of interest. If the fragment is complementary to the oligonucleotide sequence on the gold nanoparticles, particles are assembled together. As a result of this “aggregation” the color of the solution changes from red to blue because the surface plasmon is coupled when particles are in the aggregated state. The color change thus indicates a positive detection. Mutations can be detected by heating the sample. DNA de-hybridizes when heated and when a mutation is present in the sequence, the melting temperature is lowered. By measuring and comparing the temperature of a mutated sequence to a perfectly complementary sequence, one is able to detect whether a mutation is present or not. More complex schemes can also be designed to identify the location of a mutation within the DNA fragment analyzed.

     

    In vivo cancer application

    The gold nanoparticle PEG/BRM system can be designed to selectively target tumors and bind to cancerous cells. Interestingly,PEGylated gold nanoparticles can target tumors by a passive mechanism alone. An advantage of the protective PEG-layer on the gold nanoparticles is that it has been shown to reduce macrophage uptake. Macrophages are part of the reticuloendothelial system that removes foreign materials from the blood. The PEGylated layer reduces the interaction of the gold surface with blood proteins thereby minimizing non-specific macrophage uptake. This allows the nanoparticles to reside in the blood for long-term, which allows for a greater chance of tumor extravasation. If coated with a drug or imaging agent, the gold nanoparticles can be used as a visualization tool as well as a delivery vehicle to the tumor. Another method of targeting tumors is by coating the gold nanoparticles with a bio-recognition molecule that recognizes receptors on tumor cells, the extracellular matrix, or blood vessels, i.e. active targeting. The advantage of using gold nanoparticles is that one can control the delivery efficiency by the size, shape, or surface chemistry (4).

    Nanotoxicology

    One of the key questions facing researchers is to understand how the size, shape, and surface chemistry (known as the physico-chemical properties) affect how nanoparticles distribute in the cell and body, and whether specific nanomaterial designs cause toxicity. Gold nanoparticles are ideal platforms to perform these studies on because they can be synthesized and tuned with a narrow size distribution. Further, designs with different shapes and surface chemistries can be readily achieved. This allows one to systematically evaluate their behavior in biological systems. By performing such studies, one is then able to identify the designs with the lowest toxicity, which subsequently can be selected for the final application(s).

    Cellular probes

    Gold nanoparticles scatter light, and when using a dark field microscope to image them, they appear as bright spots (similar to fluorescence). A unique advantage is that the dark field signal does not photobleach like fluorescence from dyes. By labeling gold nanoparticles with bio-recognition molecules such as an antibody to a cell surface receptor etc. cells can thus conveniently be targeted and imaged. In addition, low-level target detection can be improved by using silver enhancement of bound gold nanoparticles.

     


    References

    1. Sperling, R. A.; Gil, P. R.;Zhang, F.; Zanella, M.; Parak, W. J.. Biological Applications of Gold Nanoparticles, Chemical Society Reviews, 2008, 37, 1896-1908.
    2. Walkey, C. D.; Olsen, J. B.; Guo, H.; Emili, A.; Chan, W. C. W. Nanoparticle Size and Surface Chemistry Determine Serum Protein Adsorption and Macrophage Uptake, Journal of the American Chemical Society, 2012, 134, 2139.
    3. Elghanian, R; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.,; Mirkin, C. A. Selective Colorimetric Detection of Polynucleotides Based on the Distance Dependent Optical Properties of Gold Nanoparticles, Science, 1997, 277, 1078.
    4. Chou, L; Chan, W. C.W. Fluorescence-Tagged Gold Nanoparticles for Rapidly Characterizing the Size-Dependent Biodistribution in Tumor Models, Advanced Healthcare Materials, 2012, DOI: 10.1002/adhm.201200084.

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    Silver nanoparticles (colloidal silver) have unique optical, electronic, and antibacterial properties, and are widely used in areas such as biosensing, photonics, electronics, and antimicrobial applications.

    Most applications in biosensing and detection exploit the optical properties of silver nanoparticles, as conferred by the localized surface plasmon resonance effect. That is, a specific wavelength (frequency) of incident light can induce collective oscillation of the surface electrons of silver nanoparticles. The particular wavelength of the localized surface plasmon resonance is dependant on the silver nanoparticle size, shape, and agglomeration state.

    Figure 1 shows the plasmon resonance spectra of different sizes of silver nanoparticles, as measured with UV-visible spectroscopy. As the particle size increases from 10 to 100 nm, the absorbance peak (lambda max) increases from 400 nm to 500 nm, and broadens in width. For particles of greater size, especially above 80 nm, a secondary peak at lower wavelength becomes apparent, which is a result of quadrupole resonance, in addition to the primary dipole resonance.

    silver nanoparticle absorbance

    Figure 1. Silver nanoparticle size dependant surface plasmon resonance. Note the red-shift of the absorption maximum as the gold nanoparticle size increases.

    The aggregation state of silver nanoparticles also has significant effect on their optical properties. This fact can be used to monitor the stability of silver nanoparticles, both over time, and upon addition of salt-containing buffers, which at high enough concentrations cause particle aggregation. As shown in the absorbance spectra in Figure 2, aggregation is indicated by a decrease in the primary peak, and an increase in the red region.

    silver nanoparticles aggregation

    Figure 2. Visual appearance and UV-VIS spectra of monodisperse (A) and sodium chloride (NaCl) induced agglomeration (B) of 10nm silver nanoparticles.

    Comparison of Silver Nanoparticles vs. Gold Nanoparticles

    Both silver nanoparticles and gold nanoparticles are commonly employed in optical detection for their surface plasmon resonance effect. The plasmon excitation efficiency of silver nanoparticles is known to be even more pronounced than that of gold nanoparticles, as shown in their stronger, sharper plasmon resonance peaks at the same particle concentration. Silver nanoparticles thus can render better sensitivity for some applications, such as localized surface plasmon resonance or surface enhanced Raman scattering detection.

    Silver nanoparticles may also be advantageous over gold nanoparticles, when used in combination with fluorescence emission detection. Most fluorophores emits at a wavelength above 500 nm. However, the plasmon resonance absorbance of gold nanoparticles is primarily in the range of 500-600 nm, and hence can quench the detectable fluorescence to some extent, when the fluorescent dyes are close to the particle surface. This issue of fluorescence quenching is minimized for silver nanoparticles, as their plasmon resonance absorbance is mostly below 500 nm, with little overlapping with the emission wavelength of most fluorescent dyes.

    With their different wavelengths of plasmon resonance, silver and gold nanoparticles, or particles of different size or shapes, can also be used together for multiplexed detection, taking advantage of the extended range of detection spectrum. Figure 4 shows dark field microscope images of silver nanoparticles, gold nanoparticles and gold nano-urchins.

    silver nanoparticle microscopy immuno dot-blot noble metal nanoparticle protein conjugates

    Figure 4. This image highlights the difference in appearance between silver nanoparticles, gold nanoparticles and gold nanourchins in darkfield microscopy (top) and in a immuno-dot blot assay (bottom).

    Summary of Silver Nanoparticles Optical and Physical Specifications at Different Sizes





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    Storage:

    Store silver nanoparticles at 2-8°C and protected from light. Do NOT freeze. If frozen, the silver nanoparticles will irreversibly aggregate. Aggregation of silver nanoparticles is indicated by change in color of the solution and an increase in the absorption of light in the red part of the visible spectrum, as detailed in Silver Nanoparticle Properties Tech Note.

    When stored as specified, the colloidal silver is stable for at least one year.
     

    Handling:

    Large silver nanoparticles, i.e., above 60 nm in size, might sediment over time but will not affect the performance of the silver nanoparticles. Prior to use, re-suspend the sedimented particles by swirling the container until a homogenous solution is obtained.

    For optimal performance and stability of the product, silver nanoparticles should not be stored diluted from the original concentration or in a bottle or flask other than that the product is supplied in. Care should be taken to use clean storage containers if other than supplied with the product.
     

    Washing:

    Although it is not always necessary, washing of silver nanoparticles might be required for certain applications. Silver ions released from the silver nanoparticle surface may affect some applications. The easiest way to remove such possible contaminants from the silver nanoparticle solution is by centrifugation. Centrifugal force is dependent on the size of silver nanoparticles, as listed Table I.
     

    Note:

    1. Surfactant should be present during centrifugation, to prevent the nanoparticles from aggregation.
    2. Since non-functionalized silver nanoparticles are sensitive to salt containing buffers, re-suspension should always be performed in ultra-pure water or 2 mM sodium citrate solution, to prevent irreversible aggregation.

    Procedure:

    1. Aliquot 1 ml of silver nanoparticle stock solution into 1.5 ml centrifuge tubes.
    2. Add Tween 20 to reach a final concentration of 0.025% (w/v).
    3. Centrifuge the silver nanoparticles for 30 minutes using appropriate G force, as shown in Table I below.
    4. Remove supernatant and re-suspend in desired volume of ultra-pure water or 2 mM sodium citrate solution.
    5. Vortex to re-disperse particles.
    6. Repeat as necessary.

    Table I.Appropriate G force for the centrifugation of silver nanoparticles. The conditions are based on centrifuging 1 mL of silver nanoparticles in a 1.5 ml microcentrifuge tubes using a standard microcentrifuge.

    Size (nm) Speed (g) Time (min)
    10* 21,000 60
    20 17,000 30
    30 11,000 30
    40 3,000 30
    50 1,800 30
    60 900 30
    80 500 30
    100 300 30

    *For 10nm silver nanoparticles, recovery was about 50% at the specified condition. For better recovery, 1) use an ultracentrifuge to achieve higher G force; 2) use a Centricon Spin Column.


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    Silver nanoparticle conjugates are useful for a wide range of biological applications including bio-sensing and bio-imaging. Stable silver nanoparticle conjugates can be prepared by two general approaches, passive adsorption and covalent bonding.

    Passive adsorption of a protein molecule onto silver nanoparticles is mediated by the electrostatic and hydrophobic interactions between the protein molecule and the surface layer of the colloidal silver. This process is maximally achieved at a pH close to the pI of the protein to be conjugated. Another important parameter is the amount of protein loading. If surface-adsorbed protein is not sufficient, aggregation occurs upon addition of electrolytes present in standard buffers. Therefore, a titration is required to determine the protein concentration for complete saturation and shielding of the silver nanoparticle surface.

    However, some proteins may undergo disturbances to the tertiary structure upon passive adsorption, which may impair their affinity and/or specificity in molecular binding applications. To better preserve the activity of the conjugated protein an alternative approach can be used, i.e., covalently coupling protein molecules onto silver nanoparticles functionalized with carboxyl groups via a PEG-linker. The PEG-linker allows for more flexibility and also better accessibility of the conjugated protein to its antigen/substrate due to the inherent mobility of the PEG-linker and the increased distance from the gold surface. Further, the shielding polyethylene glycol (PEG) layer generally also result in conjugates with superior stability and less non-specific binding. Conjugation to these types of functionalized particles can be performed using straightforward carbodiimide (EDC/NHS) coupling chemistry.
     

    Conjugation of Proteins Using a Passive Adsorption Method

    Material and Equipment Required

    Standard Silver Nanoparticles
    10% NaCl(w/v)
    10% Tween 20(w/v)
    2mM sodium citrate tribasic dihydrate
    10X Phosphate Buffered Saline, PBS
    Bovine Serum Albumin (BSA)
    UV-VIS Spectrophotometer

    Step 1. Determination of Optimal pH and Protein Concentration for Conjugation

    1. Aliquot 200 μl of silver nanoparticles into 1.5ml Eppendorf tubes (200 μl for each condition to be tested).
    2. Adjust the pH of the silver nanoparticle solution to the desired pH (optimal pH is generally close to the pI of the protein to be conjugated).
    3. Add between 0 and 50 μg of protein in 10 μl to the silver nanoparticles and mix well to determine the amount needed to saturate the silver surface.
    4. Incubate for 10 minutes at room temperature
    5. Add 200 μl of a 10% NaCl stock solution and incubate for 10 minutes at room temperature.
    6. Determine at which protein concentration the silver nanoparticle surface becomes saturated and no aggregation occurs upon addition of 10% NaCl by observing the color change and measuring the sample(s) using a UV-VIS spectrophotometer. Degree of aggregation can be measured by an increase in absorbance at 690nm and a decrease in absorbance at 405-480nm (particle size dependant, see silver nanoparticle properties) compared to that of the non-conjugated control particles.

    Note: The amount of protein needed to saturate the silver colloid can also be determined and verified through agarose gel-electroporesis. Binding of protein to the silver nanoparticle surface changes the overall particle charge and size both of which will affect the migration pattern in the agarose gel.


    Scale-Up and Preparation of Final Silver Nanoparticle Conjugate

    1. Transfer the desired volume of silver nanoparticles to 1.5 ml Eppendorf tubes.
    2. Add Tween 20 to a final concentration of 0.025% (w/v).
    3. Centrifuge the solution to pellet the silver nanoparticles. For more information on appropriate centrifugation settings for silver nanoparticles of different sizes, see table I at the end of this document.
    4. Resuspend the silver nanoparticles with 2mM sodium citrate to the original silver colloid volume and concentration.
    5. Adjust the pH of the silver nanoparticle solution as determined in the titration procedure above.
    6. Add the appropriate amount of protein as determined in the titration procedure above plus an additional 10%.
    7. Incubate for 60 minutes at room temperature on a rotary shaker/rocker.
    8. Centrifuge the vial for 30 minutes at the appropriate speed for the silver nanoparticle size that you are conjugating to pellet the particles and remove the supernatant.
    9. Resuspend the pellet in 1XPBS supplemented with 1% BSA (w/v).
    10. Sonicate briefly in a sonicator bath to aid in dispersion if particles are partially agglomerated.
    11. Validate the functionality of the final silver conjugate.
    12. Store the silver conjugate at 4 degrees Celsius until use.

    ----------------------

    Covalent Conjugation of Proteins to Carboxylated Silver Nanoparticles


    Materials and Equipment Required

    Carboxyl Functional Silver Nanoparticles (conc. 125 OD in ddH2O)
    1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC)
    N-hydroxysulfosuccinimide (Sulfo-NHS)
    Protein to be conjugated (1mg/ml in 1XPBS)
    Bovine Serum Albumin (BSA)
    Activation Buffer: 10mM 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5
    Coupling Buffer: 1X Phosphate Buffered Saline (1XPBS)
    Washing Buffer: 1X Phosphate Buffered Saline, 0.05% Tween 20 (PBST)


    Conjugation Procedure

    1. Prepare a fresh EDC/NHS solution in activation buffer at a concentration of 30 and 60 mg/ml, respectively.
    2. Transfer 10 µl of carboxyl silver nanoparticles from stock solution and mix with 10 µL of EDC/NHS solution.
    3. Incubate for 30 minutes at room temperature to activate the particle surface.
    4. Add 1 ml of washing buffer and mix thoroughly.
    5. Pellet the activated silver nanoparticles by centrifugation for 30 minutes at the appropriate speed for the particular silver nanoparticle size you are conjugating. See table I below for recommended centrifugation speeds.
    6. Remove supernatant taking care not to disturbe the pellet.
    7. Add 10 µl of protein to be conjugated (1 mg/ml in 1X PBS)
    8. Incubate for 2-4 hours on a rotary shaker/rocker to conjugate the protein to the activated silver nanoparticle surface.
    9. Add 1 ml of PBST and mix thoroughly.
    10. Pellet the silver conjugate as in step 5 above.
    11. Remove supernatant and resuspend the conjugate with 157 µl of 1X PBS supplemented with 1% BSA (w/v).
    12. Sonicate the conjugate briefly in a sonicator bath to aid in dispersion.
    13. Validate the functionality of the final silver conjugate.
    14. Store the silver conjugate at 4 degrees Celsius until use.
    immuno-dot blot silver nanoparticle protein conjugate.

    Figure 1.Validation of functionality (biotin binding) of a streptavidin silver nanoparticle conjugate using an immuno-dot blot assay.

    -------------

    Table I. Recommended centrifugation speeds for silver nanoparticles of different sizes. Note that centrifugation times and speeds are based on a 1 ml sample volume in 1.5 ml microcentrifuge tubes.

    Silver Nanoparticle Diameter (nm) Centrifugation Force Time (min)
    10nm 21,000 x g 60
    20nm 17,000 x g 30
    30nm 11,000 x g 30
    40nm 3,000 x g 30
    50nm 1,500 x g 30
    60nm 900 x g 30
    70nm 700 x g 30
    80nm 500 x g 30
    90nm 400 x g 30
    100nm 300 x g 30

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    Spectrophotometry allows for the quantitative measurement of molecular compounds or substances by measuring the quantitative reflection or transmission properties of matter as a function of a specific wavelength. For example, oligonucleotides are quantified by measuring the absorbance of ultraviolet light (UV) of a given oligonucleotide sample using a spectrophotometer.

    One optical density unit, or 1 O.D., typically equals 50 pg/ml of double stranded DNA; 33 pg/ml of single stranded DNA; or 40 pg/ml of single stranded RNA.

    Usually, automated synthesis of oligonucleotides is performed at scales described in micromoles. Standard synthesis protocols can range from 200 nanomoles to 50 µmoles per oligonucleotide synthesized.

    The scale of a synthesis can be converted to units of weight (microgram, milligram, or gram) by using the following formula:

    The average molecular weight of a deoxynucleotide base or 1-mer, is 325,

    Therefore,

    1 µmol of a 25-mer oligonucleotide = 325 x 25 g / 106 = 0.081 g = 8.1mg

    And,

    8.1 mg = 8100 µg / 33 = 245 O.D. (for single stranded DNA)

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    Peptide linker is the most common attachment method in bio-conjugation 1-3 and between various biopolymers. That approach requires one biopolymer or a surface having an amino function and another having an activated carboxylate.


    Oligonucleotide conjugation is predominantly carried out by use of a nucleophilic group on an oligonucleotide to react with an electrophilic group on a reporter molecule or a solid support.  This is predominant approach because the common oligonucleotide deprotection is performed by base treatment e.g. ammonia, primary alkylamines or their combinations which are inherently nucleophilic.  However, there are many situations when researchers need to introduce an electrophilic group into oligonucleotides and use it in the attachment method towards of a nucleophilic moiety.


    In the case, when oligonucleotides have been used in that type of the bio-conjugation the activated carboxylate have been generated post-synthetically.2  Free carboxyl modified oligonucleotides can be activated by EDC in situ in the organic or aqueous conditions and subsequently conjugated to aminated counterpart (Fig. 1).  However, in order to generate free carboxylate attached oligonucleotide using commercially available building blocks it requires cleavage of the ester bond with alkaline base4,5 before oligonucleotide base deprotection in concentrated ammonia, otherwise the ester will be converted into corresponding amide.

     

    Figure 1.

     carboxyl modified oligonucleotidescarboxyl modified oligonucleotides

     

    Using that strategy carboxyl modified oligonucleotides can be easily immobilized on solid support such as micro-array slides and various types of aminoalkylated beads.

    Bio-synthesis offers not only wide varieties of 3’-, 5’- and internally carboxyl modified oligonucleotides and also their conjugates with peptides, proteins and antibodies.1

     

    References:

    1.      http://www.biosyn.com/Bioconjugation.aspx

    2.       E. Jablonski, E. W.Moomaw, R. H.Tullis and J. L.Ruth Nucleic Acid Res., 1986, 14, 6115-6128.

    3.      J. D. Kahl and M. M. Greenberg  J. Org. Chem., 1999, 64 (2), 507–510

    4.      A.V. Kachalova, T. S. Zatsepin, E. A. Romanova, D. A. Strelenko, M. J. Gait, T. S. Oretskaya Nucleosides, Nucleotides Nucleic Acids. 2000, 19, 1693-1707

    5.      T. P. Prakash, A. M. Kawasaki, E. A. Lesnik, S. R. Owens, M. Manoharan Org. Lett. 2003, 5, 403-406.

     

     


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    By Klaus D. Linse

    Delta sleep inducing peptide, or DSIP, is a nonapeptide with the sequence WAGGDASGE and a molecular weight of 849 dalton. The peptide was discovered by G. Schoeneberger and M. Monnier in 1974. Results from different research groups indicated that DSIP plays an important role in stress-resistance since DSIP showed stress-protective, antiseizure, and immunomodulating effects. Furthermore, it has been found to also play a role in slowing down the aging process. The peptide mainly induces delta-sleep in rabbits, rats, mice, and humans. However, in cats, the effect on REM sleep was found to be more pronounced. Using radio immune assays (RIA) and immunohistochemistry it was possible for researchers to localize DSIP-like material in the brain and in peripheral organs of the rat as well as in the plasma of several mammals. In addition, the peptide has also been observed to affect electrophysiological activity, neurotransmitter levels in the brain, circadian and locomotor patterns, hormonal levels, psychological performance, and the activity of neuro-pharmacological drugs including their withdrawal. DSIP influences the nocturnal rise of N-acetyltransferase (NAT) activity in the rat pineal gland. The peptide is now known to have multifunctional regulatory properties and numerous studies have confirmed its stress-protective and adaptive activity. Apparently the exact biochemical origin of this peptide is not well known, however, in humans, the sequence is present in the Jumonji domain-containing protein 1B or JMJD1B protein, in lysine-specific demethylase 3B, and the KIAA1082 protein as can be revealed by a BLAST search of the human genome. The JMJD1B protein, encoded by JMJD1B gene, is a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3. The protein appears to play a central role in histone regulation. The demethylation of Lys residue generates formaldehyde and succinate. In addition this protein may have tumor suppressor activity. The KIAA1082 protein belongs to a protein domain family that is part of the cupin metallo-enzyme superfamily, smart00558, which includes a JmjC domain encoded by the KIAA1082 gene. This neuropeptide when infused into the mesodiencephalic ventricle of recipient rabbits induces spindle and delta electro-encephalography (EEG) activity and reduced motor activities. Electroencephalography (EEG) refers to the recording of electrical activity along the scalp and measures voltage fluctuations resulting from ionic current flows within the neurons of the brain.

    Another peptide called “delta sleep-inducing immunoreactive peptide (DIP)” is a 77-residue NH2-terminally acetylated peptide that was originally isolated from porcine brain (pDIP) using polyclonal antibodies against the delta sleep-inducing peptide (DSIP). As reported by Seidel et al in 1997 pDIP was detected via DSIP-specific antibodies. It is thought that these antibodies may have recognized the sequence GGDA in DSIP and GGSA in pDIP. However the peptide is otherwise not sequence-related to this supposed sleep-inducing peptide. The pDIP sequence contains a putative leucine-zipper motif, a Pro/Glu rich domain, and three potential phosphorylation sites. The human analog of pDIP as characterized by cDNA analysis showed that human DIP (hDIP) differs from the porcine protein in only four residues. Specifically, Arg55 of pDIP is changed to a cysteine in hDIP. Furthermore, hDIP was shown to be present in a large number of tissues by reverse transcription polymerase chain reaction/Southern hybridization analysis.
     
    The TSC22 domain family protein 3 isoform 3 found in humans, also called “delta sleep inducing peptide, immunoreactor” is a TSC-22 related protein. The amino acid sequence is also present in the DSIP-immunoreactive leucine zipper protein and the glucocorticoid-induced leucine zipper protein. These peptides and proteins belong to the TSC22 domain protein family 3. Other names referred to in databases are TSC-22-like protein, TSC-22-related protein, DSIP-immunoreactive peptide, and delta sleep-inducing peptide immunoreactor. The calculated molecular weight is 8,542 dalton. The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs gene product. Both proteins are leucine zipper proteins that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10. It appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine and transcript variants encoding different isoforms have also been identified for this gene. [Source RefSeq, Jul 2008].


    Delta-Sleep Inducing Peptides (DSIP) and Analogs

    Table for Delta Sleep Inducing Peptide
     

     
    Structural models of the Delta-Sleep Inducing Peptide (DSIP) and Analogs
     

    Delta-Sleep Inducing Peptide (DSIP)

    Delta Sleep Inducing Peptide


     
    Chemical structure of DSIP

    Chemical structure of DSIP
    Chemical structure of DSIP

    Energy minimized Van-der-Waals model of DSIP using a simple force field modeling approach.


     
    (Asn5)-Delta-Sleep Inducing Peptide
     
    ((Asn5)-Delta-Sleep Inducing Peptide (rabbit); (Asn5)-DSIP (rabbit))
    Asn5 Delta Sleep Inducing Peptide


    Chemical structure of (Asn5)-DSIP
    Chemical structure of Asn5 DSIP
    Energy minimized Van-der-Waals model of (Asn5)-DSIP using a simple force field modeling approach.
     


    (β-Asp5)-Delta-Sleep Inducing Peptide
     
    H-Trp-Ala-Gly-Gly-Asp(Ala-Ser-Gly-Glu-OH)-OH
     
    Asp5 Delta Sleep Inducing Peptide
    Asp5 Delta Sleep Inducing Peptide
    (β-Asp5)-DSIP: Energy minimized Van-der-Waals model using a simple force field modeling approach.
     
    This peptide analog is a good substrate for protein L-isoaspartyl methyltransferase, an enzyme found in the brain which is involved in the repair of age-damaged proteins containing atypical iosaspartyl peptide bonds.


     
    Porcine Delta-Sleep-Inducing Peptide Immunoreactive Peptide (pDIP)
     
    The solution structure for this peptide was determined by Seidel et al. in 1997.
    Porcine Delta Sleep Inducing Peptide Immunoreactive Peptide pDIP

    pDIP: Representation of the three-dimensional model of pDIP (Seidel et al 1997).


     
    References
     
    Backmund M, Meyer K, Rothenhaeusler HB and Soyka M (1998). "Opioid detoxification with delta sleep-inducing peptide: results of an open clinical trial.". J. Clin. Psychopharmacol. 18: 257–258.
     
    Bjartell A, Ekman R, Sundler F and Widerlöv E (1988). "Delta sleep-inducing peptide (DSIP): An overview of central actions and possible relationship to psychiatric illnesses". Nordic Journal of Psychiatry 42: 111–117.
     
    Charnay Y, Bouras C, Vallet PG, Golaz J, Guntern R, Constantinidis J (1989). "Immunohistochemical distribution of delta sleep inducing peptide in the rabbit brain and hypophysis". Neuroendocrinology 49: 169–175.
     
    Charnay Y, Golaz J, Vallet PG, Bouras C (1992). "Production and immunohistochemical application of monoclonal antibodies against delta sleep-inducing peptide". J Chem Neuroanat 5: 503–9.
     
    Friedman TC, García-Borreguero D, Hardwick D, Akuete CN, Doppman JL, Dorn LD, Barker CN, Yanovski JA, Chrousos GP (1994). "Decreased Delta-Sleep and Plasma Delta-Sleep-Inducing Peptide in Patients with Cushing Syndrome". Clinical Neuroendocrinology 60: 626–634.
     
    M.V.Graf and A.J.Kastin, Neurosci. Biobehav. Rev. 8, 83 (1984)1 M. V.Graf and A.J.Kastin, Peptides 7, 1165 (1986)
     
    Graf MV, Kastin AJ.; Delta-sleep-inducing peptide (DSIP): a review. Neurosci Biobehav Rev. 1984 Spring;8(1):83-93.
     
    M.V. Graf, G.A. Schoenenberger; DSIP affects adrenergic stimulation of rat pineal N-acetyltransferase in vivo and in vitro. Volume 7, Issue 6, November–December 1986, Pages 1001–1006.
     
    Graf MV, Kastin AJ.; Delta-sleep-inducing peptide (DSIP): an update. Peptides. 1986 Nov-Dec;7(6):1165-87.
     
    Gimble JM, Ptitsyn AA, Goh BC, Hebert T, Yu G, Wu X, Zvonic S, Shi X-M and Floyd ZE (2009). "Delta sleep-inducing peptide and glucocorticoidinduced leucine zipper: potential links between circadian mechanisms and obesity?". Obesity reviews 10: 46–51.
     
    Gupta V, Awasthi N and Wagner BJ (2007). "Specific Activation of the Glucocorticoid Receptor and Modulation of Signal Transduction Pathways in Human Lens Epithelial Cells". Investigative Ophthalmology and Visual Science 48: 1724–1734.
     
     
     
     
    B.A. Johnson and D.W.Aswad, Neurochem. Res. r8, 87 (1993)
     
    Iyer KS, Marks GA, Kastin AJ, and McCann SM (1988). "Evidence for a role of delta sleep-inducing peptide in slow-wave sleep and sleep-related growth hormone release in the rat". Proc Natl Acad Sci USA 85: 3653–3656.
     
    Khvatova EM, Samartzev VN, Zagoskin PP, Prudchenko IA, Mikhaleva II (2003). "Delta sleep inducing peptide (DSIP): effect on respiration activity in rat brain mitochondria and stress protective potency under experimental hypoxia". Peptides 24: 307–311.
     
    Kovalzon VM (1994). "DSIP: a sleep peptide or unknown hypothalamic hormone?". J. Evol. Biochem. Physiol. 30: 195–199.
     
    Kovalzon VM (2001). "Sleep-Inducing Properties of DSIP Analogs: Structural and Functional Relationships". Biology Bulletin 28: 394–400.
     
    Kovalzon VM, Strekalova TV; Delta sleep-inducing peptide (DSIP): a still unresolved riddle. J Neurochem. 2006 Apr; 97(2):303-9.
     
    Nakagaki K, Ebihara S, Usui S, Honda Y, Takahashi Y, Kato N (1986). "Effects of intraventricular injection of anti-DSIP serum on sleep in rats". Yakubutsu Seishin Kodo (Japanese journal of psychopharmacology) 6: 259–65.
     
    Nakamura A, Nakashima M, Sugao T, Kanemoto H, Fukumura Y, Shiomi H (1988). "Potent antinociceptive effect of centrally administered delta-sleep-inducing peptide (DSIP)". Eur J Pharmacol 155: 247–53.
     
    Pollard BJ and Pomfrett CJD (2001). "Delta sleep-inducing peptide".  Eur. J. Anaesthesiol. 18: 419–422.
     
    Pomfrett CJD, Dolling S, Anders NRK, Glover DG, Bryan A, Pollard BJ (2009). "Delta sleep-inducing peptide alters bispectral index, the electroencephalogram and heart rate variability when used as an adjunct to isoflurane anaesthesia". Eur J Anaesthesiol 26: 128–34.
     
    Irina G. Popovich, Boris O. Voitenkov, Vladimir N. Anisimov, Vadim T. Ivanov, Inessa I. Mikhaleva, Mark A. Zabezhinski, Irina N. Alimova, Dmitri A. Baturin a, Natalia Yu. Zavarzina, Svetlana V. Rosenfeld, Anna V. Semenchenko, Anatoli I. Yashin; Effect of delta-sleep inducing peptide-containing preparation Deltaran on biomarkers of aging, life span and spontaneous tumor
    incidence in female SHR mice. Mechanisms of Ageing and Development 124 (2003) 721-731.
     
    Schneider-Helmert D (1986). "DSIP in Sleep Disturbances". Eur Neurol 25: 154–157.

    Schneider-Helmert D and Schoenenberger GA (1981). "The influence of synthetic DSIP (delta-sleep-inducing-peptide) on disturbed human sleep". Cellular and Molecular Life Sciences 37: 913–917.
     
    Schoenenberger GA, Maier PF, Tobler HJ and Monnier M (1977). "A naturally occurring delta-EEG enhancing nonapeptide in rabbits". European Journal of Physiology 369: 99–109.
     
    Schoenenberger GA (1984). "Characterization, properties and multivariate functions of Delta-Sleep Inducing Peptide (DSIP)". European Neurology 23: 321–345.
    G.A.Schoenenberger and M.Monnier, Proc. Nat. Acad. Sci. USA 74, 1282 (1977)
     
    Gabi Seidel, Knut Adermann, Thomas Schindler, Andrzej Ejchart, Rainer Jaenicke, Wolf-Georg Forssmann, Paul Roesch; Solution Structure of Porcine Delta Sleep-inducing Peptide Immunoreactive Peptide A Homolog of the Shortsighted Gene Product.  THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 272, No. 49, Issue of December 5, pp. 30918–30927, 1997.
     
    Seifritz E, Muller M, Schonenberger G, Trachsel L, Hemmeter U, Hatzinger M, Ernst A, Moore P and Holsboer-Trachsler E (1995). "Human plasma DSIP decreases at the initiation of sleep at different circadian times". Peptides 16: 1475–1481.

    Shi X, Shi W, Li Q, Song B, Wan M, Bai S (2003). "A glucocorticoid-induced leucine-zipper protein, GILZ, inhibits adipogenesis of mesenchymal cells". EMBO Rep 4: 374–380.

    Sinyukhin AB, Timoshinov GP, Komilov VA, Shabanov PD (2009). "Delta sleep-inducing peptide analogue corrects the eNS functional state of children treated with antiblastomic therapy". European Neuropsychopharmacology 19: S681.

    Soyka M and Rothenhaeusler H (1997). "Delta Sleep-Inducing Peptide Opioid Detoxification". Am. J. Psychiat. 154: 714–715.
     
    Steiger A and Holsboer F (1997). "Neuropeptides and human sleep". Sleep 20: 1038–1052.
     
    Stanojilovic OP, Zivanovic DP and Su Sic VT (2002). "The effects of Delta Sleep-Inducing Peptide on incidence and severity in metaphit-induced epilepsy in rats". Pharmacological Research 45: 241–247.

    Stanojlovic OP, Zivanovic DP, Mirkovic S, Mikhaleva II (2004). "Delta sleep-inducing peptide and its tetrapeptide analogue alleviate severity of metaphit seizures". Pharmacology, Biochemistry and Behavior 77: 227–234.
     
    Sudakova KV, Umriukhina PE, Rayevskyb KS (2004). "Delta-sleep inducing peptide and neuronal activity after glutamate microiontophoresis: the role of NMDA-receptors". Pathophysiology 11: 81–86.
     
    Sudakova KV, Coghlan JP, Kotov AV, Salieva RM, Polyntsev YV, Koplik EV (1995). "Delta-sleep inducing peptide sequels in mechanisms of resistance to emotional stress". Ann. N.Y. Acad. Sci. 771: 240–251.
     
    Susić V, Masirević G, Totić S (1987). "The effects of delta-sleep-inducing peptide (DSIP) on wakefulness and sleep patterns in the cat". Brain Research 414: 262–70.
     
    Walleus H, Widerlöv E and Ekman R (1985). "Decreased concentrations of delta-sleep inducing peptide in plasma and cerebrospinal fluid from depressed patients". Nordic Journal of Psychiatry 39: 63–67.
     
    Westrin A, Ekman R, and Traskman-Bendz L (1998). "High Delta Sleep-Inducing Peptide-Like Immunoreactivity in Plasma in Suicidal Patients with Major Depressive Disorder". Biological Psychiatry 43: 734–739.
     
    Yehuda S, Kastin AJ and Coy DH (1980). "Thermoragulatory and locomotor effects of DSIP: paradoxical interaction with d-amphetamine". Pharmacol. Biochem. Behav. 13: 895–900.
     
    Yehuda S and Mostofsky DI (1984). "Modification of the hypothermic circadian cycles induced by DSIP and melatonin in pinealectomized and hypophysectomised rats". Peptides 5: 495–497.

    Yehuda S and Carasso R (1988). "DSIP – a tool for investigating the sleep onset mechanism: a review". International J. Neurosci. 38: 345–353.

    Yukhananov RY, Tennila TML, Miroshnichenko II, Kudrina VS, Ushakov AN and Melnik EI (1992). "Ethanol and Delta Sleep Inducing Peptide effects on brain monoamines". Pharmacol. Bio-chem. Behav. 43: 683–687.



      

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